LOCUS       pCS2+_mScarletI3-NbALFA_boomUTR        5649 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  16.
ACCESSION   urn.local...st-klr9ygh
VERSION     urn.local...st-klr9ygh
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             164..>859
                     /note="Red fluorescent reporter protein - Ex:569, Em:593"
                     /product="mScarlet-I"
                     /gene="mScarlet-I"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mScarlet-I"
     misc_feature    853^854
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TAA"
     gene            869..1240
                     /standard_name="optZF_NbALFA"
     3'UTR           1262..1584
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1675..1693)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1711..1832
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1858..1890)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1944..1963)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2002..2029)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2008..2024
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2033..2062)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2040..2051
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2077..2098
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(2386..2974)
     CDS             complement(3145..4005)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(4006..4110)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     rep_origin      complement(4136..4591)
     regulatory      4663..5649
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg atagcaccga
      181 agcagtgatc aaggagttca tgcggttcaa ggtgcacatg gaaggctcaa tgaatggcca
      241 cgagttcgag atcgagggtg agggcgaggg acgaccctac gagggcacgc aaaccgctaa
      301 gctgaaggtc accaagggtg gccccctgcc cttctcctgg gacatcctgt ccccacagtt
      361 catgtacggt tccagagcct ttatcaagca ccctgctgac atcccagact actggaagca
      421 gtccttccct gagggattca agtgggagcg tgtgatgatc ttcgaggacg gcggaaccgt
      481 gtccgtgacc caggatacat ccctggagga cggaaccctg atctacaagg tgaagctccg
      541 tggcggcaat ttcccacctg atggacctgt aatgcaaaag agaaccatgg gctgggaagc
      601 atctaccgag cgtctgtacc ccgaggatgt cgtgctgaag ggcgacatca agatggctct
      661 gcgcctgaag gacggcggcc gctacctggc ggatttcaag acaacctaca aggctaagaa
      721 gcccgtccag atgcccggag ctttcaacat cgatcgtaag ttggacatca cctcccataa
      781 cgaggactac accgtcgtgg aacagtacga acgttccgtt gctcgccact ctaccggtgg
      841 ctccggtgga tccaggtgtg gtcgacatat ggatgaagtc cagctgcagg agtccggtgg
      901 tggcctggtt caaccaggtg gctccctacg actgtcctgt accgcatccg gagttacgat
      961 tagcgctctg aatgctatgg caatgggatg gtatcgtcag gcaccgggag agcgtcgtgt
     1021 catggtagct gctgtctctg aacgtggaaa tgctatgtat cgtgaatcag ttcagggccg
     1081 ttttaccgtt acccgcgatt tcaccaacaa aatggtcagt ctgcagatgg acaacctgaa
     1141 accggaggat acagcggtgt actactgcca tgtcctggaa gatcgcgttg atagcttcca
     1201 cgattattgg ggacagggga cccaggtcac tgtgtcctca taatacgtag attaagagct
     1261 ctctcaaaat gaggaatgag caatgatttt ttaaagaaaa attttacgag ttggatttta
     1321 tttcactggt gaacggagtg cgcggctaca ttgaggcttc gagatggtgg tcatgcgcca
     1381 cagacaaatg tagtttcagt cactgtttgg ctaacttatt ccacttgggc tgtctgttta
     1441 gcggcttgcc ttgtttatta cattattttg ctaccaaagg ttttattgtg aattgccaat
     1501 gttttcatga atcaagtttg cgtcatcttt aaatagttgt ttagacattt taaatgttca
     1561 ttgttaataa aaataactaa acaactagaa tacatacttc taatcattcc atagctaata
     1621 catacttcta atcattccat agctaataca tacttctaat cattccatac tagaactata
     1681 gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca
     1741 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1801 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1861 ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt
     1921 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     1981 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2041 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2101 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2161 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2221 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2281 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2341 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2401 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2461 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2521 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2581 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2641 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2701 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2761 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2821 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2881 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2941 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3001 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3061 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     4141 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4201 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4261 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4321 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4381 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4441 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4501 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4561 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     4621 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccat agccaattca
     4681 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
     4741 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
     4801 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg
     4861 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
     4921 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
     4981 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
     5041 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
     5101 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
     5161 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
     5221 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
     5281 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
     5341 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
     5401 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
     5461 gacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
     5521 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
     5581 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
     5641 ttagggaac
//
