LOCUS       pCS2+_mScarlet-OptZF_NbVHH05_boomUTR        5625 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  22.
ACCESSION   urn.local...ni-klr98lb
VERSION     urn.local...ni-klr98lb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             170..>862
                     /note="Red fluorescent reporter protein - Ex:569, Em:594"
                     /product="mScarlet"
                     /gene="mScarlet"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mScarlet"
     gene            878..1216
                     /standard_name="optZF_VHH05"
     3'UTR           1238..1560
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1651..1669)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1687..1808
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1834..1866)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1920..1939)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1978..2005)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1984..2000
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2009..2038)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2016..2027
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2053..2074
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(2362..2950)
     CDS             complement(3121..3981)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(3982..4086)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     rep_origin      complement(4112..4567)
     regulatory      4639..5625
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggccgtg atcaaagaat tcatgcgctt caaggtgcac atggaaggca gcatgaacgg
      241 acacgagttc gagatcgaag gcgaaggcga gggcagacct tacgagggaa cacagaccgc
      301 caagctgaaa gtgaccaaag gcggacctct gcctttcagc tgggacatcc tgtctccaca
      361 gtttatgtac ggaagccgcg ccttcacaaa gcaccctgct gacatccctg actactacaa
      421 gcagagcttc cctgagggat tcaagtggga gagagtgatg aacttcgagg acggcggagc
      481 cgtgacagtg acacaggata ccagcctgga agatggaacc ctgatctaca aagtgaagct
      541 gcgcggaaca aacttccctc ctgacggacc tgtgatgcag aaaaagacca tgggatggga
      601 agccagcacc gagagactgt accctgaaga tggcgtgctg aagggcgaca tcaagatggc
      661 cctgagactg aaagacggcg gaagatacct ggccgacttc aagaccacct acaaggccaa
      721 gaaacctgtg cagatgcctg gcgcctacaa cgtggacaga aagctggaca tcaccagcca
      781 caacgaggac tacaccgtgg tggaacagta cgagagaagc gagggacgcc acagcacagg
      841 cggaatggac gagctgtaca agaggtgtgg tcgacatatg gatcaggtgc agctgcagga
      901 gtcaggcggc ggactggtgc agcctggagg gtctctacgt ctgtcctgtg ctgcctctgg
      961 attcgtcttc gagaattcgg ccatggcctg gtaccgtcag gctcctggaa aggagcgtga
     1021 actgatcgct gttattggta cgacctttat aaagcttgct gagtccgtga agggacgatt
     1081 caccatctcc cgtgataatg ccaagagtac ggtctacctg caaatgaata atctgaagcc
     1141 tgaggacacc gccgtgtatt actgttcaaa gtctggcgcc tactggggac agggaaccca
     1201 ggtcaccgtc tcctcataat acgtagatta agagctctct caaaatgagg aatgagcaat
     1261 gattttttaa agaaaaattt tacgagttgg attttatttc actggtgaac ggagtgcgcg
     1321 gctacattga ggcttcgaga tggtggtcat gcgccacaga caaatgtagt ttcagtcact
     1381 gtttggctaa cttattccac ttgggctgtc tgtttagcgg cttgccttgt ttattacatt
     1441 attttgctac caaaggtttt attgtgaatt gccaatgttt tcatgaatca agtttgcgtc
     1501 atctttaaat agttgtttag acattttaaa tgttcattgt taataaaaat aactaaacaa
     1561 ctagaataca tacttctaat cattccatag ctaatacata cttctaatca ttccatagct
     1621 aatacatact tctaatcatt ccatactaga actatagtga gtcgtattac gtagatccag
     1681 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1741 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1801 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1861 aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc
     1921 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1981 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2041 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2101 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2161 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2761 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3061 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3121 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3181 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3241 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3301 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3361 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3421 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3481 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3541 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3601 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3661 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3721 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3781 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3841 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3901 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3961 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4021 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4081 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     4141 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4201 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4261 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4321 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4381 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4441 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4501 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4561 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4621 tcttcgctat tacgccagtc gaccatagcc aattcaatat ggcgtatatg gactcatgcc
     4681 aattcaatat ggtggatctg gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc
     4741 aattcaatat ggtggatctg gaccccagcc aattcaatat ggcggacttg gcaccatgcc
     4801 aattcaatat ggcggacttg gcactgtgcc aactggggag gggtctactt ggcacggtgc
     4861 caagtttgag gaggggtctt ggccctgtgc caagtccgcc atattgaatt ggcatggtgc
     4921 caataatggc ggccatattg gctatatgcc aggatcaata tataggcaat atccaatatg
     4981 gccctatgcc aatatggcta ttggccaggt tcaatactat gtattggccc tatgccatat
     5041 agtattccat atatgggttt tcctattgac gtagatagcc cctcccaatg ggcggtccca
     5101 tataccatat atggggcttc ctaataccgc ccatagccac tcccccattg acgtcaatgg
     5161 tctctatata tggtctttcc tattgacgtc atatgggcgg tcctattgac gtatatggcg
     5221 cctcccccat tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc
     5281 aataggacca cccaccattg acgtcaatgg gatggctcat tgcccattca tatccgttct
     5341 cacgccccct attgacgtca atgacggtaa atggcccact tggcagtaca tcaatatcta
     5401 ttaatagtaa cttggcaagt acattactat tggaaggacg ccagggtaca ttggcagtac
     5461 tcccattgac gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca tccccattga
     5521 cgtcaatggg gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg
     5581 cgtgcctaat gggaggtcta tataagcaat gctcgtttag ggaac
//
