LOCUS       pCS2+_mScarlet-Nb127d01_boomUTR        5611 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  28.
ACCESSION   urn.local...nj-klr98lb
VERSION     urn.local...nj-klr98lb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           108..>140
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    107^108
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="G"
     misc_feature    141..143
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TCAACTTTGGCAGATCGCCGCCACC"
     CDS             144..>836
                     /note="Red fluorescent reporter protein - Ex:569, Em:594"
                     /product="mScarlet"
                     /gene="mScarlet"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mScarlet"
     misc_feature    230..244
                     /note="polyarginine tag used for protein purification (may
                     sometime have 6 R residues instead of 5)"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="polyArg"
     misc_feature    <837..851
                     /note="Geneious type: ORF"
                     /standard_name="fbxw11b"
     gene            852..1202
                     /standard_name="optZF_Nb127d01"
     primer_bind     1215..1236
                     /standard_name="Cterm fusion F"
     3'UTR           1224..1546
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1637..1655)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1673..1794
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1820..1852)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1906..1925)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1964..1991)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1970..1986
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(1995..2024)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2002..2013
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2039..2060
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2333..2952)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3107..3967)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(3968..4072)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4009..4037)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4230..4536)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4625..5611
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttctt gttctttttg
      121 cagaagctca gaataaacgc atggtgagca agggcgaggc cgtgatcaaa gaattcatgc
      181 gcttcaaggt gcacatggaa ggcagcatga acggacacga gttcgagatc gaaggcgaag
      241 gcgagggcag accttacgag ggaacacaga ccgccaagct gaaagtgacc aaaggcggac
      301 ctctgccttt cagctgggac atcctgtctc cacagtttat gtacggaagc cgcgccttca
      361 caaagcaccc tgctgacatc cctgactact acaagcagag cttccctgag ggattcaagt
      421 gggagagagt gatgaacttc gaggacggcg gagccgtgac agtgacacag gataccagcc
      481 tggaagatgg aaccctgatc tacaaagtga agctgcgcgg aacaaacttc cctcctgacg
      541 gacctgtgat gcagaaaaag accatgggat gggaagccag caccgagaga ctgtaccctg
      601 aagatggcgt gctgaagggc gacatcaaga tggccctgag actgaaagac ggcggaagat
      661 acctggccga cttcaagacc acctacaagg ccaagaaacc tgtgcagatg cctggcgcct
      721 acaacgtgga cagaaagctg gacatcacca gccacaacga ggactacacc gtggtggaac
      781 agtacgagag aagcgaggga cgccacagca caggcggaat ggacgagctg tacaagaggt
      841 gtggtcgaca tatggatgag gtccaacttg tggaatctgg cggaggactg gttcaagccg
      901 gtgagtcctt gcgtttgagc tgcgctgctt ctgggagcac ctttgatttc aaggtgatgg
      961 gctggtatcg tcagccgcct ggaaagcagc gtgagggtgt agctgccatc cgcctgtctg
     1021 gaaatatgca ctacgctgag tctgtcaaag ggcgctttgc tatttccaaa gctaacgcta
     1081 agaatacagt ctaccttcaa atgaattcat tgcgtcctga ggataccgct gtttattact
     1141 gcaaagtcaa catccgtggg caggattact ggggtcaagg aacgcaggtc accgtctctt
     1201 cttaatacgt agattaagag ctctctcaaa atgaggaatg agcaatgatt ttttaaagaa
     1261 aaattttacg agttggattt tatttcactg gtgaacggag tgcgcggcta cattgaggct
     1321 tcgagatggt ggtcatgcgc cacagacaaa tgtagtttca gtcactgttt ggctaactta
     1381 ttccacttgg gctgtctgtt tagcggcttg ccttgtttat tacattattt tgctaccaaa
     1441 ggttttattg tgaattgcca atgttttcat gaatcaagtt tgcgtcatct ttaaatagtt
     1501 gtttagacat tttaaatgtt cattgttaat aaaaataact aaacaactag aatacatact
     1561 tctaatcatt ccatagctaa tacatacttc taatcattcc atagctaata catacttcta
     1621 atcattccat actagaacta tagtgagtcg tattacgtag atccagacat gataagatac
     1681 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1741 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     1801 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc
     1861 gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt
     1921 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2101 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2521 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2761 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3001 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3061 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3241 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4081 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4141 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4201 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4261 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4321 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4381 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4441 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4501 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     4561 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     4621 ccagtcgacc atagccaatt caatatggcg tatatggact catgccaatt caatatggtg
     4681 gatctggacc tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg
     4741 gatctggacc ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg
     4801 gacttggcac tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg
     4861 ggtcttggcc ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc
     4921 atattggcta tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata
     4981 tggctattgg ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat
     5041 gggttttcct attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg
     5101 ggcttcctaa taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt
     5161 ctttcctatt gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac
     5221 gtcaattacg gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca
     5281 ccattgacgt caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg
     5341 acgtcaatga cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg
     5401 gcaagtacat tactattgga aggacgccag ggtacattgg cagtactccc attgacgtca
     5461 atggcggtaa atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg
     5521 ggcaatgacg caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga
     5581 ggtctatata agcaatgctc gtttagggaa c
//
