LOCUS       pCS2+_mScarlet-FbHA_boomUTR        6034 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  3.
ACCESSION   urn.local...6o-klr5olc
VERSION     urn.local...6o-klr5olc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             171..>863
                     /note="Red fluorescent reporter protein - Ex:569, Em:594"
                     /product="mScarlet"
                     /gene="mScarlet"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mScarlet"
     CDS             864..1640
                     /standard_name="HA Frankenbody (15F11)"
     misc_feature    863^864
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     misc_feature    1637^1638
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGAGGTTCTGGAGGAGGAGGTTCGGGAGGTGGTGGA
                     TCAGGAGGCGGCGGAAGCGGTGGAGGAGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1647..1969
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2060..2078)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2096..2217
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2243..2275)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2329..2348)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2387..2414)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2393..2409
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2418..2447)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2425..2436
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2462..2483
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2756..3375)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3530..4390)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4391..4495)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4432..4460)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4653..4959)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5048..6034
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gtgagcaagg
      181 gcgaggccgt gatcaaagaa ttcatgcgct tcaaggtgca catggaaggc agcatgaacg
      241 gacacgagtt cgagatcgaa ggcgaaggcg agggcagacc ttacgaggga acacagaccg
      301 ccaagctgaa agtgaccaaa ggcggacctc tgcctttcag ctgggacatc ctgtctccac
      361 agtttatgta cggaagccgc gccttcacaa agcaccctgc tgacatccct gactactaca
      421 agcagagctt ccctgaggga ttcaagtggg agagagtgat gaacttcgag gacggcggag
      481 ccgtgacagt gacacaggat accagcctgg aagatggaac cctgatctac aaagtgaagc
      541 tgcgcggaac aaacttccct cctgacggac ctgtgatgca gaaaaagacc atgggatggg
      601 aagccagcac cgagagactg taccctgaag atggcgtgct gaagggcgac atcaagatgg
      661 ccctgagact gaaagacggc ggaagatacc tggccgactt caagaccacc tacaaggcca
      721 agaaacctgt gcagatgcct ggcgcctaca acgtggacag aaagctggac atcaccagcc
      781 acaacgagga ctacaccgtg gtggaacagt acgagagaag cgagggacgc cacagcacag
      841 gcggaatgga cgagctgtac aagatggccg aggtgaagct ggtggagtct ggaggaggcc
      901 tggtgaagcc tggaggatcc ctgaagctct cctgtgctgc ctcgggattc actttctcct
      961 catacggcat gtcttgggtt agacagactc cggagaagcg tcttgagtgg gtcgccacta
     1021 ttagtcgagg tggtagttac acctactatc cagacagtgt gaaggggcga ttcaccatct
     1081 cccgtgacaa tgccaagaac accctgtatc tgcagatgag ctctctgcgt tctgaggata
     1141 cggccatcta ttactgtgca cgacgtgaaa catatgatga aaagggcttt gcctactggg
     1201 gccaaggcac cactctcaca gtctcctcag gtggaggcgg atcaggcgga ggtggatctg
     1261 gcggtggagg atcggatatc gtgctgaccc aatccccagc ttctttgact gtgtctctgg
     1321 ggcagagggc caccatctcc tgcaaatcca gccaaagtct gctgaatagt ggaaatcaga
     1381 agaactacct cacctggtac caacagaaac caggacagcc tccaaagctc ctcatctact
     1441 gggcatccac tcgtgaatcc gggattccag ctaggttcag tggcagtggg tctgggacag
     1501 acttcaccct caacatccat cctgtggagg aggaagatgc tgctacctat tactgtcaga
     1561 acgataattc ccatccgctt acgttcggtg ctgggaccaa gctggaaata aagagagcgg
     1621 cagccaaggg cgaattctaa gagctctctc aaaatgagga atgagcaatg attttttaaa
     1681 gaaaaatttt acgagttgga ttttatttca ctggtgaacg gagtgcgcgg ctacattgag
     1741 gcttcgagat ggtggtcatg cgccacagac aaatgtagtt tcagtcactg tttggctaac
     1801 ttattccact tgggctgtct gtttagcggc ttgccttgtt tattacatta ttttgctacc
     1861 aaaggtttta ttgtgaattg ccaatgtttt catgaatcaa gtttgcgtca tctttaaata
     1921 gttgtttaga cattttaaat gttcattgtt aataaaaata actaaacaac tagaatacat
     1981 acttctaatc attccatagc taatacatac ttctaatcat tccatagcta atacatactt
     2041 ctaatcattc catactagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     2101 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2161 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2221 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2281 ttcgcggccg cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag
     2341 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2581 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2701 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2761 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2821 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2881 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2941 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3001 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3061 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3121 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3181 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3241 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3301 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3361 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3421 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3541 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3601 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3661 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3721 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3781 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3841 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3901 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3961 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4021 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4081 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4141 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4381 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4501 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4561 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4621 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4681 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4741 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4801 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4861 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4921 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     4981 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5041 acgccagtcg accatagcca attcaatatg gcgtatatgg actcatgcca attcaatatg
     5101 gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg
     5161 gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg
     5221 gcggacttgg cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg
     5281 aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg
     5341 gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca
     5401 atatggctat tggccaggtt caatactatg tattggccct atgccatata gtattccata
     5461 tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata
     5521 tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt ctctatatat
     5581 ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt
     5641 gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac
     5701 ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta
     5761 ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac
     5821 ttggcaagta cattactatt ggaaggacgc cagggtacat tggcagtact cccattgacg
     5881 tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg
     5941 aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg
     6001 ggaggtctat ataagcaatg ctcgtttagg gaac
//
