LOCUS       pCS2+_mScarlet-FbFLAG_boomUTR        6025 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  9.
ACCESSION   urn.local...f6-klr7e2w
VERSION     urn.local...f6-klr7e2w
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     misc_feature    103
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    168..170
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     CDS             171..>863
                     /note="Red fluorescent reporter protein - Ex:569, Em:594"
                     /product="mScarlet"
                     /gene="mScarlet"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mScarlet"
     misc_feature    257..271
                     /note="polyarginine tag used for protein purification (may
                     sometime have 6 R residues instead of 5)"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="polyArg"
     primer_bind     complement(843..863)
                     /standard_name="mScarlet (696 R) - pCS2+ OptZF_FbFLAG
                     boomUTR PCR Product"
     CDS             864..1628
                     /standard_name="FbFLAG"
     misc_feature    863^864
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     misc_feature    864..878
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     864..884
                     /standard_name="FbFLAG VecF"
     misc_feature    <864..1647
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="gblock (concatenated sequence 2)"
     primer_bind     complement(1612..1628)
                     /standard_name="gblock (765 R) - pCS2+eGFP-OptZF_NbALFA
                     boomUTR PCR Product"
     misc_feature    1628^1629
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGCGGTTCTGGGGGTGGAGGTAGTGGAGGTGGAGGT
                     TCAGGAGGCGGAGGAAGCGGTGGAGGTGGAAGCGCCTCCCTCCCAGCC"
     misc_feature    1629..1647
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     1629..1650
                     /standard_name="Cterm fusion F"
     misc_feature    <1629..1647
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pCS2+zGrad OptZF_NbALFA PCR Product
                     (concatenated sequence 2)"
     3'UTR           1638..1960
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2051..2069)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2087..2208
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2234..2266)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2320..2339)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2378..2405)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2384..2400
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2409..2438)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2416..2427
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2453..2474
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2747..3366)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3521..4381)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4382..4486)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4423..4451)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4644..4950)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5039..6025
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gtgagcaagg
      181 gcgaggccgt gatcaaagaa ttcatgcgct tcaaggtgca catggaaggc agcatgaacg
      241 gacacgagtt cgagatcgaa ggcgaaggcg agggcagacc ttacgaggga acacagaccg
      301 ccaagctgaa agtgaccaaa ggcggacctc tgcctttcag ctgggacatc ctgtctccac
      361 agtttatgta cggaagccgc gccttcacaa agcaccctgc tgacatccct gactactaca
      421 agcagagctt ccctgaggga ttcaagtggg agagagtgat gaacttcgag gacggcggag
      481 ccgtgacagt gacacaggat accagcctgg aagatggaac cctgatctac aaagtgaagc
      541 tgcgcggaac aaacttccct cctgacggac ctgtgatgca gaaaaagacc atgggatggg
      601 aagccagcac cgagagactg taccctgaag atggcgtgct gaagggcgac atcaagatgg
      661 ccctgagact gaaagacggc ggaagatacc tggccgactt caagaccacc tacaaggcca
      721 agaaacctgt gcagatgcct ggcgcctaca acgtggacag aaagctggac atcaccagcc
      781 acaacgagga ctacaccgtg gtggaacagt acgagagaag cgagggacgc cacagcacag
      841 gcggaatgga cgagctgtac aagatggccg aggtgaagct ggtggaatct ggaggaggat
      901 tagtgaagcc tggaggttcc ctgaagctct catgcgcagc ctctggattc accttcagta
      961 gctttggaat gcattgggtt agacagactc cggagaagcg tcttgagtgg gtagcataca
     1021 ttagtagtgg cagtagtacc atctactatg cagacaccgt gaagggccga ttcaccatct
     1081 cccgtgacaa tgccaagaac acgctgtacc tgcaaatgag cagtctgagg tctgaggaca
     1141 cggctattta ttactgtgct agatcccttg ctaccgccgc ttttgcttac tggggacaag
     1201 gcaccactct caccgtttcc tcaggtggag gcggttcagg aggtggaggc tctggcggtg
     1261 gcggatcgga cattgtgctg acccaatctc cagcatctct gactgtgtct ctagggcaga
     1321 gggccaccat ctcctgccgt tctagtcaga gtattgtcta tagtaatggt aacacctacc
     1381 ttgaatggta ccaacagaag ccaggacagc cacccaaact cctgatctat aaggtttcta
     1441 accgattttc cggaatccca gccaggttca gtggcagtgg gtctgggaca gacttcaccc
     1501 tgaacattca tcctgtggag gaggaggatg ctgctaccta ttactgcttt cagggatcac
     1561 atgttccgta cacattcggt gctggcacca agctggaaat aaaacgggct gccgccaagg
     1621 gcgagttcta agagctctct caaaatgagg aatgagcaat gattttttaa agaaaaattt
     1681 tacgagttgg attttatttc actggtgaac ggagtgcgcg gctacattga ggcttcgaga
     1741 tggtggtcat gcgccacaga caaatgtagt ttcagtcact gtttggctaa cttattccac
     1801 ttgggctgtc tgtttagcgg cttgccttgt ttattacatt attttgctac caaaggtttt
     1861 attgtgaatt gccaatgttt tcatgaatca agtttgcgtc atctttaaat agttgtttag
     1921 acattttaaa tgttcattgt taataaaaat aactaaacaa ctagaataca tacttctaat
     1981 cattccatag ctaatacata cttctaatca ttccatagct aatacatact tctaatcatt
     2041 ccatactaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     2101 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2161 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2221 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     2281 gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     2341 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2401 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2461 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2521 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3181 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4501 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4561 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4621 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4681 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4741 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4801 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4861 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4921 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     4981 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc
     5041 gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg
     5101 gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg
     5161 gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacttg
     5221 gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt
     5281 ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg
     5341 gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta
     5401 ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt
     5461 tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc
     5521 ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc
     5581 tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat
     5641 tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg
     5701 acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca
     5761 atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt
     5821 acattactat tggaaggacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg
     5881 gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat
     5941 gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta
     6001 tataagcaat gctcgtttag ggaac
//
