LOCUS       pCS2+_mNG-NbVHH05_boomUTR        5610 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  21.
ACCESSION   urn.local...oq-klr9cc1
VERSION     urn.local...oq-klr9cc1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..>847
                     /note="Green fluorescent reporter protein - Ex:506,
                     Em:517"
                     /product="mNeonGreen"
                     /gene="mNeonGreen"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mNeonGreen"
     misc_feature    <848..862
                     /note="Geneious type: ORF"
                     /standard_name="fbxw11b"
     gene            863..1201
                     /standard_name="optZF_VHH05"
     misc_feature    1211..>1213
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
                     /standard_name="SV40 poly(A) signal"
     3'UTR           1223..1545
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1636..1654)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1672..1793
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1819..1851)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1905..1924)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1963..1990)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1969..1985
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(1994..2023)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2001..2012
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2038..2059
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(2347..2935)
     CDS             complement(3106..3966)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(3967..4071)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4008..4036)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     rep_origin      complement(4097..4552)
     regulatory      4624..5610
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cctccctccc
      181 agccacccac gagctccaca tcttcggatc catcaacgga gtcgacttcg acatggtcgg
      241 acaaggaacc ggaaacccaa acgacggata cgaggagctc aacctcaagt ccaccaaggg
      301 agacctccaa ttctccccat ggatcctcgt cccacacatc ggatacggat tccaccaata
      361 cctcccatac ccagacggaa tgtccccatt ccaagccgcc atggtcgacg gatccggata
      421 ccaagtccac cgtaccatgc aattcgagga cggagcctcc ctcaccgtca actaccgtta
      481 cacctacgag ggatcccaca tcaagggaga ggcccaagtc aagggaaccg gattcccagc
      541 cgacggacca gtcatgacca actccctcac cgccgccgac tggtgccgtt ccaagaagac
      601 ctacccaaac gacaagacca tcatctccac cttcaagtgg tcctacacca ccggaaacgg
      661 aaagcgttac cgttccaccg cccgtaccac ctacaccttc gccaagccaa tggccgccaa
      721 ctacctcaag aaccaaccaa tgtacgtctt ccgtaagacc gagctcaagc actccaagac
      781 cgagctcaac ttcaaggagt ggcaaaaggc cttcaccgac gtcatgggaa tggacgagct
      841 ctacaagagg tgtggtcgac atatggatca ggtgcagctg caggagtcag gcggcggact
      901 ggtgcagcct ggagggtctc tacgtctgtc ctgtgctgcc tctggattcg tcttcgagaa
      961 ttcggccatg gcctggtacc gtcaggctcc tggaaaggag cgtgaactga tcgctgttat
     1021 tggtacgacc tttataaagc ttgctgagtc cgtgaaggga cgattcacca tctcccgtga
     1081 taatgccaag agtacggtct acctgcaaat gaataatctg aagcctgagg acaccgccgt
     1141 gtattactgt tcaaagtctg gcgcctactg gggacaggga acccaggtca ccgtctcctc
     1201 ataatacgta gattaagagc tctctcaaaa tgaggaatga gcaatgattt tttaaagaaa
     1261 aattttacga gttggatttt atttcactgg tgaacggagt gcgcggctac attgaggctt
     1321 cgagatggtg gtcatgcgcc acagacaaat gtagtttcag tcactgtttg gctaacttat
     1381 tccacttggg ctgtctgttt agcggcttgc cttgtttatt acattatttt gctaccaaag
     1441 gttttattgt gaattgccaa tgttttcatg aatcaagttt gcgtcatctt taaatagttg
     1501 tttagacatt ttaaatgttc attgttaata aaaataacta aacaactaga atacatactt
     1561 ctaatcattc catagctaat acatacttct aatcattcca tagctaatac atacttctaa
     1621 tcattccata ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca
     1681 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1741 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1801 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg
     1861 cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt
     1921 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4081 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4141 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4201 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4261 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4321 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4381 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4441 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4501 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     4561 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4621 cagtcgacca tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg
     4681 atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg
     4741 atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg
     4801 acttggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
     4861 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
     4921 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
     4981 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
     5041 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
     5101 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
     5161 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
     5221 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
     5281 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
     5341 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
     5401 caagtacatt actattggaa ggacgccagg gtacattggc agtactccca ttgacgtcaa
     5461 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
     5521 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
     5581 gtctatataa gcaatgctcg tttagggaac
//
