LOCUS       pCS2+_mNG-NbALFA_boomUTR        5643 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  14.
ACCESSION   urn.local...oo-klr9cc1
VERSION     urn.local...oo-klr9cc1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..>847
                     /note="Green fluorescent reporter protein - Ex:506,
                     Em:517"
                     /product="mNeonGreen"
                     /gene="mNeonGreen"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mNeonGreen"
     gene            863..1234
                     /standard_name="optZF_NbALFA"
     misc_feature    1244..>1246
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
                     /standard_name="SV40 poly(A) signal"
     3'UTR           1256..1578
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1669..1687)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1705..1826
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1852..1884)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1938..1957)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1996..2023)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2002..2018
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2027..2056)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2034..2045
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2071..2092
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2365..2984)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3139..3999)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4000..4104)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4041..4069)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4262..4568)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4657..5643
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cctccctccc
      181 agccacccac gagctccaca tcttcggatc catcaacgga gtcgacttcg acatggtcgg
      241 acaaggaacc ggaaacccaa acgacggata cgaggagctc aacctcaagt ccaccaaggg
      301 agacctccaa ttctccccat ggatcctcgt cccacacatc ggatacggat tccaccaata
      361 cctcccatac ccagacggaa tgtccccatt ccaagccgcc atggtcgacg gatccggata
      421 ccaagtccac cgtaccatgc aattcgagga cggagcctcc ctcaccgtca actaccgtta
      481 cacctacgag ggatcccaca tcaagggaga ggcccaagtc aagggaaccg gattcccagc
      541 cgacggacca gtcatgacca actccctcac cgccgccgac tggtgccgtt ccaagaagac
      601 ctacccaaac gacaagacca tcatctccac cttcaagtgg tcctacacca ccggaaacgg
      661 aaagcgttac cgttccaccg cccgtaccac ctacaccttc gccaagccaa tggccgccaa
      721 ctacctcaag aaccaaccaa tgtacgtctt ccgtaagacc gagctcaagc actccaagac
      781 cgagctcaac ttcaaggagt ggcaaaaggc cttcaccgac gtcatgggaa tggacgagct
      841 ctacaagagg tgtggtcgac atatggatga agtccagctg caggagtccg gtggtggcct
      901 ggttcaacca ggtggctccc tacgactgtc ctgtaccgca tccggagtta cgattagcgc
      961 tctgaatgct atggcaatgg gatggtatcg tcaggcaccg ggagagcgtc gtgtcatggt
     1021 agctgctgtc tctgaacgtg gaaatgctat gtatcgtgaa tcagttcagg gccgttttac
     1081 cgttacccgc gatttcacca acaaaatggt cagtctgcag atggacaacc tgaaaccgga
     1141 ggatacagcg gtgtactact gccatgtcct ggaagatcgc gttgatagct tccacgatta
     1201 ttggggacag gggacccagg tcactgtgtc ctcataatac gtagattaag agctctctca
     1261 aaatgaggaa tgagcaatga ttttttaaag aaaaatttta cgagttggat tttatttcac
     1321 tggtgaacgg agtgcgcggc tacattgagg cttcgagatg gtggtcatgc gccacagaca
     1381 aatgtagttt cagtcactgt ttggctaact tattccactt gggctgtctg tttagcggct
     1441 tgccttgttt attacattat tttgctacca aaggttttat tgtgaattgc caatgttttc
     1501 atgaatcaag tttgcgtcat ctttaaatag ttgtttagac attttaaatg ttcattgtta
     1561 ataaaaataa ctaaacaact agaatacata cttctaatca ttccatagct aatacatact
     1621 tctaatcatt ccatagctaa tacatacttc taatcattcc atactagaac tatagtgagt
     1681 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     1741 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1801 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     1861 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     1921 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     1981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2821 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     4141 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4201 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4261 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4321 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4381 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4441 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4501 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4561 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     4621 gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa ttcaatatgg
     4681 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
     4741 cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg
     4801 cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa ctggggaggg
     4861 gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat
     4921 attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata
     4981 taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt
     5041 attggcccta tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc
     5101 tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc atagccactc
     5161 ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc
     5221 ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc
     5281 tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg
     5341 cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg
     5401 gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg gaaggacgcc
     5461 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
     5521 caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg
     5581 acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg
     5641 aac
//
