LOCUS       pCS2+_mNG-Nb127d01_boomUTR        5638 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  27.
ACCESSION   urn.local...op-klr9cc1
VERSION     urn.local...op-klr9cc1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..>847
                     /note="Green fluorescent reporter protein - Ex:506,
                     Em:517"
                     /product="mNeonGreen"
                     /gene="mNeonGreen"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mNeonGreen"
     gene            879..1229
                     /standard_name="optZF_Nb127d01"
     3'UTR           1251..1573
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1664..1682)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1700..1821
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1847..1879)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1933..1952)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1991..2018)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1997..2013
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2022..2051)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2029..2040
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2066..2087
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2360..2979)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3134..3994)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(3995..4099)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4036..4064)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4257..4563)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4652..5638
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cctccctccc
      181 agccacccac gagctccaca tcttcggatc catcaacgga gtcgacttcg acatggtcgg
      241 acaaggaacc ggaaacccaa acgacggata cgaggagctc aacctcaagt ccaccaaggg
      301 agacctccaa ttctccccat ggatcctcgt cccacacatc ggatacggat tccaccaata
      361 cctcccatac ccagacggaa tgtccccatt ccaagccgcc atggtcgacg gatccggata
      421 ccaagtccac cgtaccatgc aattcgagga cggagcctcc ctcaccgtca actaccgtta
      481 cacctacgag ggatcccaca tcaagggaga ggcccaagtc aagggaaccg gattcccagc
      541 cgacggacca gtcatgacca actccctcac cgccgccgac tggtgccgtt ccaagaagac
      601 ctacccaaac gacaagacca tcatctccac cttcaagtgg tcctacacca ccggaaacgg
      661 aaagcgttac cgttccaccg cccgtaccac ctacaccttc gccaagccaa tggccgccaa
      721 ctacctcaag aaccaaccaa tgtacgtctt ccgtaagacc gagctcaagc actccaagac
      781 cgagctcaac ttcaaggagt ggcaaaaggc cttcaccgac gtcatgggaa tggacgagct
      841 ctacaaggga cgagctgtac aagaggtgtg gtcgacatat ggatgaggtc caacttgtgg
      901 aatctggcgg aggactggtt caagccggtg agtccttgcg tttgagctgc gctgcttctg
      961 ggagcacctt tgatttcaag gtgatgggct ggtatcgtca gccgcctgga aagcagcgtg
     1021 agggtgtagc tgccatccgc ctgtctggaa atatgcacta cgctgagtct gtcaaagggc
     1081 gctttgctat ttccaaagct aacgctaaga atacagtcta ccttcaaatg aattcattgc
     1141 gtcctgagga taccgctgtt tattactgca aagtcaacat ccgtgggcag gattactggg
     1201 gtcaaggaac gcaggtcacc gtctcttctt aatacgtaga ttaagagctc tctcaaaatg
     1261 aggaatgagc aatgattttt taaagaaaaa ttttacgagt tggattttat ttcactggtg
     1321 aacggagtgc gcggctacat tgaggcttcg agatggtggt catgcgccac agacaaatgt
     1381 agtttcagtc actgtttggc taacttattc cacttgggct gtctgtttag cggcttgcct
     1441 tgtttattac attattttgc taccaaaggt tttattgtga attgccaatg ttttcatgaa
     1501 tcaagtttgc gtcatcttta aatagttgtt tagacatttt aaatgttcat tgttaataaa
     1561 aataactaaa caactagaat acatacttct aatcattcca tagctaatac atacttctaa
     1621 tcattccata gctaatacat acttctaatc attccatact agaactatag tgagtcgtat
     1681 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1741 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1801 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1861 ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggcccggtac
     1921 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     1981 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2041 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2161 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3421 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3721 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     4141 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4201 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4261 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4321 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4381 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4441 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4501 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4561 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4621 atcggtgcgg gcctcttcgc tattacgcca gtcgaccata gccaattcaa tatggcgtat
     4681 atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat
     4741 atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac
     4801 ttggcaccat gccaattcaa tatggcggac ttggcactgt gccaactggg gaggggtcta
     4861 cttggcacgg tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga
     4921 attggcatgg tgccaataat ggcggccata ttggctatat gccaggatca atatataggc
     4981 aatatccaat atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg
     5041 ccctatgcca tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca
     5101 atgggcggtc ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca
     5161 ttgacgtcaa tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt
     5221 gacgtatatg gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat
     5281 gcccattgac gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat
     5341 tcatatccgt tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt
     5401 acatcaatat ctattaatag taacttggca agtacattac tattggaagg acgccagggt
     5461 acattggcag tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt
     5521 acatccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta
     5581 aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
