LOCUS pCS2+_mNG-Nb127d01_boomUTR 5638 bp DNA circular UNA 11-MAY-2026 DEFINITION 27. ACCESSION urn.local...op-klr9cc1 VERSION urn.local...op-klr9cc1 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers regulatory 34..51 /note="Geneious type: Promoter" /standard_name="Sp6 prom" 5'UTR 109..151 /note="Xenopus globin 5′ UTR sequence" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="Xenopus globin 5′ UTR" CDS 167..>847 /note="Green fluorescent reporter protein - Ex:506, Em:517" /product="mNeonGreen" /gene="mNeonGreen" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /standard_name="mNeonGreen" gene 879..1229 /standard_name="optZF_Nb127d01" 3'UTR 1251..1573 /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="prrg2 3'UTR" regulatory complement(1664..1682) /note="Geneious type: Promoter" /standard_name="T7 prom" regulatory 1700..1821 /note="Geneious type: terminator" /note="simian vacuolating virus 40 (SV40) polyA signal" /regulatory_class="terminator" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="SV40 polyA" misc_feature complement(1847..1879) /note="KT3 epitope tag used for protein detection and purification" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on protein similarity" /standard_name="KT3" regulatory complement(1933..1952) /note="Geneious type: Promoter" /standard_name="T3 prom" misc_feature complement(1991..2018) /note="Geneious type: Regulatory Sequence" /standard_name="lacO reg" misc_feature 1997..2013 /note="Lac operon operator" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="lacO" regulatory complement(2022..2051) /note="Geneious type: Promoter" /standard_name="lac prom" misc_feature 2029..2040 /note="C-tag used for protein purification" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on protein similarity" /standard_name="C" regulatory 2066..2087 /note="catabolite activator protein binding site" /regulatory_class="other" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="CAP BS" misc_feature complement(2360..2979) /note="Geneious type: Origin of Replication" /standard_name="pBR322 origin" misc_feature complement(3134..3994) /note="Geneious type: Marker" /standard_name="amp marker" regulatory complement(3995..4099) /note="Geneious type: promoter" /note="Promoter for ampicillin resistance gene (b-lactamase)" /regulatory_class="promoter" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" regulatory complement(4036..4064) /note="Geneious type: Promoter" /standard_name="amp prom" misc_feature complement(4257..4563) /note="Geneious type: Origin of Replication" /standard_name="f1 origin" regulatory 4652..5638 /note="Geneious type: promoter" /note="Human cytomegalovirus (CMV) immediate early promoter" /regulatory_class="promoter" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="CMV IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt 121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cctccctccc 181 agccacccac gagctccaca tcttcggatc catcaacgga gtcgacttcg acatggtcgg 241 acaaggaacc ggaaacccaa acgacggata cgaggagctc aacctcaagt ccaccaaggg 301 agacctccaa ttctccccat ggatcctcgt cccacacatc ggatacggat tccaccaata 361 cctcccatac ccagacggaa tgtccccatt ccaagccgcc atggtcgacg gatccggata 421 ccaagtccac cgtaccatgc aattcgagga cggagcctcc ctcaccgtca actaccgtta 481 cacctacgag ggatcccaca tcaagggaga ggcccaagtc aagggaaccg gattcccagc 541 cgacggacca gtcatgacca actccctcac cgccgccgac tggtgccgtt ccaagaagac 601 ctacccaaac gacaagacca tcatctccac cttcaagtgg tcctacacca ccggaaacgg 661 aaagcgttac cgttccaccg cccgtaccac ctacaccttc gccaagccaa tggccgccaa 721 ctacctcaag aaccaaccaa tgtacgtctt ccgtaagacc gagctcaagc actccaagac 781 cgagctcaac ttcaaggagt ggcaaaaggc cttcaccgac gtcatgggaa tggacgagct 841 ctacaaggga cgagctgtac aagaggtgtg gtcgacatat ggatgaggtc caacttgtgg 901 aatctggcgg aggactggtt caagccggtg agtccttgcg tttgagctgc gctgcttctg 961 ggagcacctt tgatttcaag gtgatgggct ggtatcgtca gccgcctgga aagcagcgtg 1021 agggtgtagc tgccatccgc ctgtctggaa atatgcacta cgctgagtct gtcaaagggc 1081 gctttgctat ttccaaagct aacgctaaga atacagtcta ccttcaaatg aattcattgc 1141 gtcctgagga taccgctgtt tattactgca aagtcaacat ccgtgggcag gattactggg 1201 gtcaaggaac gcaggtcacc gtctcttctt aatacgtaga ttaagagctc tctcaaaatg 1261 aggaatgagc aatgattttt taaagaaaaa ttttacgagt tggattttat ttcactggtg 1321 aacggagtgc gcggctacat tgaggcttcg agatggtggt catgcgccac agacaaatgt 1381 agtttcagtc actgtttggc taacttattc cacttgggct gtctgtttag cggcttgcct 1441 tgtttattac attattttgc taccaaaggt tttattgtga attgccaatg ttttcatgaa 1501 tcaagtttgc gtcatcttta aatagttgtt tagacatttt aaatgttcat tgttaataaa 1561 aataactaaa caactagaat acatacttct aatcattcca tagctaatac atacttctaa 1621 tcattccata gctaatacat acttctaatc attccatact agaactatag tgagtcgtat 1681 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 1741 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 1801 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 1861 ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggcccggtac 1921 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc 1981 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 2041 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 2101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 2161 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 2221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 2281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 2341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 2521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 2581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 2641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 2701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 2761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 2821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 3001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 3061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 3121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 3181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 3241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 3301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 3361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 3421 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 3481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 3541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 3601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 3661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 3721 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 3781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 3841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 3901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 3961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 4021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 4081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa 4141 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 4201 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 4261 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 4321 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 4381 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 4441 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 4501 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 4561 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg 4621 atcggtgcgg gcctcttcgc tattacgcca gtcgaccata gccaattcaa tatggcgtat 4681 atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat 4741 atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac 4801 ttggcaccat gccaattcaa tatggcggac ttggcactgt gccaactggg gaggggtcta 4861 cttggcacgg tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga 4921 attggcatgg tgccaataat ggcggccata ttggctatat gccaggatca atatataggc 4981 aatatccaat atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg 5041 ccctatgcca tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca 5101 atgggcggtc ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca 5161 ttgacgtcaa tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt 5221 gacgtatatg gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat 5281 gcccattgac gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat 5341 tcatatccgt tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt 5401 acatcaatat ctattaatag taacttggca agtacattac tattggaagg acgccagggt 5461 acattggcag tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt 5521 acatccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta 5581 aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac //