LOCUS       pCS2+_mNG-FbHA_boomUTR        6019 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  2.
ACCESSION   urn.local...5z-klr5nvn
VERSION     urn.local...5z-klr5nvn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             168..>848
                     /note="Green fluorescent reporter protein - Ex:506,
                     Em:517"
                     /product="mNeonGreen"
                     /gene="mNeonGreen"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mNeonGreen"
     misc_feature    848^849
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     primer_bind     849..869
                     /standard_name="FbHA Vec F"
     CDS             849..1625
                     /standard_name="HA Frankenbody (15F11)"
     misc_feature    <1623..1641
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pCS2+zGrad OptZF_NbALFA PCR Product
                     (concatenated sequence 2)"
     misc_feature    1622^1623
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGAGGTTCTGGAGGAGGAGGTTCGGGAGGTGGTGGA
                     TCAGGAGGCGGCGGAAGCGGTGGAGGAGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1632..1954
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2045..2063)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2081..2202
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2228..2260)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2314..2333)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2372..2399)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2378..2394
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2403..2432)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2410..2421
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2447..2468
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(2756..3344)
     CDS             complement(3515..4375)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(4376..4480)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4417..4445)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     rep_origin      complement(4506..4961)
     regulatory      5033..6019
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gcctccctcc
      181 cagccaccca cgagctccac atcttcggat ccatcaacgg agtcgacttc gacatggtcg
      241 gacaaggaac cggaaaccca aacgacggat acgaggagct caacctcaag tccaccaagg
      301 gagacctcca attctcccca tggatcctcg tcccacacat cggatacgga ttccaccaat
      361 acctcccata cccagacgga atgtccccat tccaagccgc catggtcgac ggatccggat
      421 accaagtcca ccgtaccatg caattcgagg acggagcctc cctcaccgtc aactaccgtt
      481 acacctacga gggatcccac atcaagggag aggcccaagt caagggaacc ggattcccag
      541 ccgacggacc agtcatgacc aactccctca ccgccgccga ctggtgccgt tccaagaaga
      601 cctacccaaa cgacaagacc atcatctcca ccttcaagtg gtcctacacc accggaaacg
      661 gaaagcgtta ccgttccacc gcccgtacca cctacacctt cgccaagcca atggccgcca
      721 actacctcaa gaaccaacca atgtacgtct tccgtaagac cgagctcaag cactccaaga
      781 ccgagctcaa cttcaaggag tggcaaaagg ccttcaccga cgtcatggga atggacgagc
      841 tctacaagat ggccgaggtg aagctggtgg agtctggagg aggcctggtg aagcctggag
      901 gatccctgaa gctctcctgt gctgcctcgg gattcacttt ctcctcatac ggcatgtctt
      961 gggttagaca gactccggag aagcgtcttg agtgggtcgc cactattagt cgaggtggta
     1021 gttacaccta ctatccagac agtgtgaagg ggcgattcac catctcccgt gacaatgcca
     1081 agaacaccct gtatctgcag atgagctctc tgcgttctga ggatacggcc atctattact
     1141 gtgcacgacg tgaaacatat gatgaaaagg gctttgccta ctggggccaa ggcaccactc
     1201 tcacagtctc ctcaggtgga ggcggatcag gcggaggtgg atctggcggt ggaggatcgg
     1261 atatcgtgct gacccaatcc ccagcttctt tgactgtgtc tctggggcag agggccacca
     1321 tctcctgcaa atccagccaa agtctgctga atagtggaaa tcagaagaac tacctcacct
     1381 ggtaccaaca gaaaccagga cagcctccaa agctcctcat ctactgggca tccactcgtg
     1441 aatccgggat tccagctagg ttcagtggca gtgggtctgg gacagacttc accctcaaca
     1501 tccatcctgt ggaggaggaa gatgctgcta cctattactg tcagaacgat aattcccatc
     1561 cgcttacgtt cggtgctggg accaagctgg aaataaagag agcggcagcc aagggcgaat
     1621 tctaagagct ctctcaaaat gaggaatgag caatgatttt ttaaagaaaa attttacgag
     1681 ttggatttta tttcactggt gaacggagtg cgcggctaca ttgaggcttc gagatggtgg
     1741 tcatgcgcca cagacaaatg tagtttcagt cactgtttgg ctaacttatt ccacttgggc
     1801 tgtctgttta gcggcttgcc ttgtttatta cattattttg ctaccaaagg ttttattgtg
     1861 aattgccaat gttttcatga atcaagtttg cgtcatcttt aaatagttgt ttagacattt
     1921 taaatgttca ttgttaataa aaataactaa acaactagaa tacatacttc taatcattcc
     1981 atagctaata catacttcta atcattccat agctaataca tacttctaat cattccatac
     2041 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
     2101 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2161 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2221 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg
     2281 ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     2341 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2401 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2461 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3181 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4501 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4561 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4621 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4681 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4741 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4801 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4861 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4921 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     4981 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccat
     5041 agccaattca atatggcgta tatggactca tgccaattca atatggtgga tctggacctg
     5101 tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc
     5161 agccaattca atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg
     5221 tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct
     5281 gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat attggctata
     5341 tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc
     5401 aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat
     5461 tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata
     5521 ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga
     5581 cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt
     5641 aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca
     5701 atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg
     5761 gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta
     5821 ctattggaag gacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat
     5881 ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca
     5941 aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag
     6001 caatgctcgt ttagggaac
//
