LOCUS       pCS2+_mNG-FbFLAG_boomUTR        6010 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  8.
ACCESSION   urn.local...ew-klr7dpw
VERSION     urn.local...ew-klr7dpw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             168..>848
                     /note="Green fluorescent reporter protein - Ex:506,
                     Em:517"
                     /product="mNeonGreen"
                     /gene="mNeonGreen"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mNeonGreen"
     CDS             849..1613
                     /standard_name="FbFLAG"
     misc_feature    848^849
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     misc_feature    1613^1614
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGCGGTTCTGGGGGTGGAGGTAGTGGAGGTGGAGGT
                     TCAGGAGGCGGAGGAAGCGGTGGAGGTGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1623..1945
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2036..2054)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2072..2193
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2219..2251)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2305..2324)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2363..2390)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2369..2385
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2394..2423)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2401..2412
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2438..2459
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2732..3351)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3506..4366)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4367..4471)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4408..4436)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4629..4935)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5024..6010
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gcctccctcc
      181 cagccaccca cgagctccac atcttcggat ccatcaacgg agtcgacttc gacatggtcg
      241 gacaaggaac cggaaaccca aacgacggat acgaggagct caacctcaag tccaccaagg
      301 gagacctcca attctcccca tggatcctcg tcccacacat cggatacgga ttccaccaat
      361 acctcccata cccagacgga atgtccccat tccaagccgc catggtcgac ggatccggat
      421 accaagtcca ccgtaccatg caattcgagg acggagcctc cctcaccgtc aactaccgtt
      481 acacctacga gggatcccac atcaagggag aggcccaagt caagggaacc ggattcccag
      541 ccgacggacc agtcatgacc aactccctca ccgccgccga ctggtgccgt tccaagaaga
      601 cctacccaaa cgacaagacc atcatctcca ccttcaagtg gtcctacacc accggaaacg
      661 gaaagcgtta ccgttccacc gcccgtacca cctacacctt cgccaagcca atggccgcca
      721 actacctcaa gaaccaacca atgtacgtct tccgtaagac cgagctcaag cactccaaga
      781 ccgagctcaa cttcaaggag tggcaaaagg ccttcaccga cgtcatggga atggacgagc
      841 tctacaagat ggccgaggtg aagctggtgg aatctggagg aggattagtg aagcctggag
      901 gttccctgaa gctctcatgc gcagcctctg gattcacctt cagtagcttt ggaatgcatt
      961 gggttagaca gactccggag aagcgtcttg agtgggtagc atacattagt agtggcagta
     1021 gtaccatcta ctatgcagac accgtgaagg gccgattcac catctcccgt gacaatgcca
     1081 agaacacgct gtacctgcaa atgagcagtc tgaggtctga ggacacggct atttattact
     1141 gtgctagatc ccttgctacc gccgcttttg cttactgggg acaaggcacc actctcaccg
     1201 tttcctcagg tggaggcggt tcaggaggtg gaggctctgg cggtggcgga tcggacattg
     1261 tgctgaccca atctccagca tctctgactg tgtctctagg gcagagggcc accatctcct
     1321 gccgttctag tcagagtatt gtctatagta atggtaacac ctaccttgaa tggtaccaac
     1381 agaagccagg acagccaccc aaactcctga tctataaggt ttctaaccga ttttccggaa
     1441 tcccagccag gttcagtggc agtgggtctg ggacagactt caccctgaac attcatcctg
     1501 tggaggagga ggatgctgct acctattact gctttcaggg atcacatgtt ccgtacacat
     1561 tcggtgctgg caccaagctg gaaataaaac gggctgccgc caagggcgag ttctaagagc
     1621 tctctcaaaa tgaggaatga gcaatgattt tttaaagaaa aattttacga gttggatttt
     1681 atttcactgg tgaacggagt gcgcggctac attgaggctt cgagatggtg gtcatgcgcc
     1741 acagacaaat gtagtttcag tcactgtttg gctaacttat tccacttggg ctgtctgttt
     1801 agcggcttgc cttgtttatt acattatttt gctaccaaag gttttattgt gaattgccaa
     1861 tgttttcatg aatcaagttt gcgtcatctt taaatagttg tttagacatt ttaaatgttc
     1921 attgttaata aaaataacta aacaactaga atacatactt ctaatcattc catagctaat
     1981 acatacttct aatcattcca tagctaatac atacttctaa tcattccata ctagaactat
     2041 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     2101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     2281 tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     2341 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2461 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3181 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     4501 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     4561 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     4621 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     4681 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4741 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4801 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4861 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4921 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     4981 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc
     5041 aatatggcgt atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc
     5101 aatatggcgt atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc
     5161 aatatggcgg acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg
     5221 gggaggggtc tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt
     5281 ccgccatatt gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat
     5341 caatatatag gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat
     5401 actatgtatt ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga
     5461 tagcccctcc caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata
     5521 gccactcccc cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg
     5581 ggcggtccta ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc
     5641 gcctggctca atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg
     5701 ctcattgccc attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc
     5761 ccacttggca gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa
     5821 ggacgccagg gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga
     5881 tggctgccaa gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt
     5941 tccattgacg taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg
     6001 tttagggaac
//
