LOCUS pCS2+_VHHgrad 5556 bp DNA circular UNA 11-MAY-2026 DEFINITION 25. ACCESSION urn.local...rn-klr9txw VERSION urn.local...rn-klr9txw KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers regulatory 34..51 /note="Geneious type: Promoter" /standard_name="Sp6 prom" 5'UTR 109..151 /note="Xenopus globin 5′ UTR sequence" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="Xenopus globin 5′ UTR" gene 167..817 /standard_name="zebrafish fbxw11b" gene 818..1156 /standard_name="optZF_VHH05" 3'UTR 1169..1491 /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="prrg2 3'UTR" regulatory complement(1582..1600) /note="Geneious type: Promoter" /standard_name="T7 prom" regulatory 1618..1739 /note="Geneious type: terminator" /note="simian vacuolating virus 40 (SV40) polyA signal" /regulatory_class="terminator" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="SV40 polyA" misc_feature complement(1765..1797) /note="KT3 epitope tag used for protein detection and purification" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on protein similarity" /standard_name="KT3" regulatory complement(1851..1870) /note="Geneious type: Promoter" /standard_name="T3 prom" misc_feature complement(1909..1936) /note="Geneious type: Regulatory Sequence" /standard_name="lacO reg" misc_feature 1915..1931 /note="Lac operon operator" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="lacO" regulatory complement(1940..1969) /note="Geneious type: Promoter" /standard_name="lac prom" misc_feature 1947..1958 /note="C-tag used for protein purification" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on protein similarity" /standard_name="C" regulatory 1984..2005 /note="catabolite activator protein binding site" /regulatory_class="other" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="CAP BS" misc_feature complement(2278..2897) /note="Geneious type: Origin of Replication" /standard_name="pBR322 origin" misc_feature complement(3052..3912) /note="Geneious type: Marker" /standard_name="amp marker" regulatory complement(3913..4017) /note="Geneious type: promoter" /note="Promoter for ampicillin resistance gene (b-lactamase)" /regulatory_class="promoter" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" regulatory complement(3954..3982) /note="Geneious type: Promoter" /standard_name="amp prom" misc_feature complement(4175..4481) /note="Geneious type: Origin of Replication" /standard_name="f1 origin" regulatory 4570..5556 /note="Geneious type: promoter" /note="Human cytomegalovirus (CMV) immediate early promoter" /regulatory_class="promoter" /note="Derived using Geneious Prime 2026.0.2 'Annotate from Database' based on nucleotide similarity" /standard_name="CMV IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt 121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat 181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg 241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc 301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg 361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat 421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca 481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt 541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg 601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc 661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac 721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat 781 agagaccatt gaagcaaact ggaggtgtgg tcgacatatg gatcaggtgc agctgcagga 841 gtcaggcggc ggactggtgc agcctggagg gtctctacgt ctgtcctgtg ctgcctctgg 901 attcgtcttc gagaattcgg ccatggcctg gtaccgtcag gctcctggaa aggagcgtga 961 actgatcgct gttattggta cgacctttat aaagcttgct gagtccgtga agggacgatt 1021 caccatctcc cgtgataatg ccaagagtac ggtctacctg caaatgaata atctgaagcc 1081 tgaggacacc gccgtgtatt actgttcaaa gtctggcgcc tactggggac agggaaccca 1141 ggtcaccgtc tcctcataat aagagctctc tcaaaatgag gaatgagcaa tgatttttta 1201 aagaaaaatt ttacgagttg gattttattt cactggtgaa cggagtgcgc ggctacattg 1261 aggcttcgag atggtggtca tgcgccacag acaaatgtag tttcagtcac tgtttggcta 1321 acttattcca cttgggctgt ctgtttagcg gcttgccttg tttattacat tattttgcta 1381 ccaaaggttt tattgtgaat tgccaatgtt ttcatgaatc aagtttgcgt catctttaaa 1441 tagttgttta gacattttaa atgttcattg ttaataaaaa taactaaaca actagaatac 1501 atacttctaa tcattccata gctaatacat acttctaatc attccatagc taatacatac 1561 ttctaatcat tccatactag aactatagtg agtcgtatta cgtagatcca gacatgataa 1621 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 1681 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 1741 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 1801 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg 1861 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 2701 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 3241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 3301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 3361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 3421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 3481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 3541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 3601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 3661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 3721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 3781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 3841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 3901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4021 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta 4081 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 4141 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 4201 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 4261 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 4321 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 4381 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 4441 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc 4501 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 4561 ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata 4621 tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata 4681 tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata 4741 tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga 4801 ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg 4861 cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc 4921 caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca 4981 tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata 5041 tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat 5101 atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca 5161 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc 5221 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc 5281 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta 5341 acttggcaag tacattacta ttggaaggac gccagggtac attggcagta ctcccattga 5401 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg 5461 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa 5521 tgggaggtct atataagcaa tgctcgttta gggaac //