LOCUS       pCS2+_VHHgrad           5556 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  25.
ACCESSION   urn.local...rn-klr9txw
VERSION     urn.local...rn-klr9txw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     gene            167..817
                     /standard_name="zebrafish fbxw11b"
     gene            818..1156
                     /standard_name="optZF_VHH05"
     3'UTR           1169..1491
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1582..1600)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1618..1739
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1765..1797)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1851..1870)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1909..1936)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1915..1931
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(1940..1969)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    1947..1958
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      1984..2005
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2278..2897)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3052..3912)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(3913..4017)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(3954..3982)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4175..4481)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4570..5556
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat
      181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg
      241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc
      301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg
      361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat
      421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca
      481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt
      541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg
      601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc
      661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac
      721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat
      781 agagaccatt gaagcaaact ggaggtgtgg tcgacatatg gatcaggtgc agctgcagga
      841 gtcaggcggc ggactggtgc agcctggagg gtctctacgt ctgtcctgtg ctgcctctgg
      901 attcgtcttc gagaattcgg ccatggcctg gtaccgtcag gctcctggaa aggagcgtga
      961 actgatcgct gttattggta cgacctttat aaagcttgct gagtccgtga agggacgatt
     1021 caccatctcc cgtgataatg ccaagagtac ggtctacctg caaatgaata atctgaagcc
     1081 tgaggacacc gccgtgtatt actgttcaaa gtctggcgcc tactggggac agggaaccca
     1141 ggtcaccgtc tcctcataat aagagctctc tcaaaatgag gaatgagcaa tgatttttta
     1201 aagaaaaatt ttacgagttg gattttattt cactggtgaa cggagtgcgc ggctacattg
     1261 aggcttcgag atggtggtca tgcgccacag acaaatgtag tttcagtcac tgtttggcta
     1321 acttattcca cttgggctgt ctgtttagcg gcttgccttg tttattacat tattttgcta
     1381 ccaaaggttt tattgtgaat tgccaatgtt ttcatgaatc aagtttgcgt catctttaaa
     1441 tagttgttta gacattttaa atgttcattg ttaataaaaa taactaaaca actagaatac
     1501 atacttctaa tcattccata gctaatacat acttctaatc attccatagc taatacatac
     1561 ttctaatcat tccatactag aactatagtg agtcgtatta cgtagatcca gacatgataa
     1621 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1681 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1741 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1801 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
     1861 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2701 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4021 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4081 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4141 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4201 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4261 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4321 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4381 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4441 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4501 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4561 ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata
     4621 tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata
     4681 tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata
     4741 tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga
     4801 ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg
     4861 cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc
     4921 caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca
     4981 tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata
     5041 tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat
     5101 atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca
     5161 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc
     5221 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc
     5281 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta
     5341 acttggcaag tacattacta ttggaaggac gccagggtac attggcagta ctcccattga
     5401 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg
     5461 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa
     5521 tgggaggtct atataagcaa tgctcgttta gggaac
//
