LOCUS       pCS2+_Sungrad_boomUTR        5974 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  33.
ACCESSION   urn.local...1cj-klrewyx
VERSION     urn.local...1cj-klrewyx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     gene            167..817
                     /standard_name="zebrafish fbxw11b"
     CDS             834..1577
                     /standard_name="FbSun (GCN4 scFv)"
     3'UTR           1587..1909
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2000..2018)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2036..2157
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2183..2215)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2269..2288)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2327..2354)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2333..2349
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2358..2387)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2365..2376
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2402..2423
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2696..3315)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3470..4330)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4331..4435)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4372..4400)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4593..4899)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4988..5974
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat
      181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg
      241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc
      301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg
      361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat
      421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca
      481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt
      541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg
      601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc
      661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac
      721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat
      781 agagaccatt gaagcaaact ggaggtgtgg tcgacatgga cgagctgtac aagatgggcc
      841 ccgacatcgt gatgacccag agccccagca gcctgagcgc cagcgtgggc gaccgcgtga
      901 ccatcacctg ccgcagcagc accggcgccg tgaccaccag caactacgcc agctgggtgc
      961 aggagaagcc cggcaagctg ttcaagggcc tgatcggcgg caccaacaac cgcgcccccg
     1021 gcgtgcccag ccgcttcagc ggcagcctga tcggcgacaa ggccaccctg accatcagca
     1081 gcctgcagcc cgaggacttc gccacctact tctgcgccct gtggtacagc aaccactggg
     1141 tgttcggcca gggcaccaag gtggagctga agcgcggcgg cggcggcagc ggcggcggcg
     1201 gcagcggcgg cggcggcagc agcggcggcg gcagcgaggt gaagctgctg gagagcggcg
     1261 gcggcctggt gcagcccggc ggcagcctga agctgagctg cgccgtgagc ggcttcagcc
     1321 tgaccgacta cggcgtgaac tgggtgcgcc aggcccccgg ccgcggcctg gagtggatcg
     1381 gcgtgatctg gggcgacggc atcaccgact acaacagcgc cctgaaggac cgcttcatca
     1441 tcagcaagga caacggcaag aacaccgtgt acctgcagat gagcaaggtg cgcagcgacg
     1501 acaccgccct gtactactgc gtgaccggcc tgttcgacta ctggggccag ggcaccctgg
     1561 tgaccgtgag cagctaataa gagctctctc aaaatgagga atgagcaatg attttttaaa
     1621 gaaaaatttt acgagttgga ttttatttca ctggtgaacg gagtgcgcgg ctacattgag
     1681 gcttcgagat ggtggtcatg cgccacagac aaatgtagtt tcagtcactg tttggctaac
     1741 ttattccact tgggctgtct gtttagcggc ttgccttgtt tattacatta ttttgctacc
     1801 aaaggtttta ttgtgaattg ccaatgtttt catgaatcaa gtttgcgtca tctttaaata
     1861 gttgtttaga cattttaaat gttcattgtt aataaaaata actaaacaac tagaatacat
     1921 acttctaatc attccatagc taatacatac ttctaatcat tccatagcta atacatactt
     1981 ctaatcattc catactagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     2041 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2101 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2161 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2221 ttcgcggccg cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag
     2281 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2341 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2401 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2461 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2521 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2581 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2641 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3121 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4441 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4501 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4561 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4621 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4681 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4741 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4801 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4861 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     4921 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4981 acgccagtcg accatagcca attcaatatg gcgtatatgg actcatgcca attcaatatg
     5041 gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg
     5101 gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg
     5161 gcggacttgg cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg
     5221 aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg
     5281 gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca
     5341 atatggctat tggccaggtt caatactatg tattggccct atgccatata gtattccata
     5401 tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata
     5461 tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt ctctatatat
     5521 ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt
     5581 gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac
     5641 ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta
     5701 ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac
     5761 ttggcaagta cattactatt ggaaggacgc cagggtacat tggcagtact cccattgacg
     5821 tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg
     5881 aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg
     5941 ggaggtctat ataagcaatg ctcgtttagg gaac
//
