LOCUS       pCS2+_Halo-NbVHH05_boomUTR        5826 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  24.
ACCESSION   urn.local...qk-klr9qcb
VERSION     urn.local...qk-klr9qcb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    <170..>1063
                     /note="HaloTag used for protein expression and
                     purification"
                     /product="haloalkane dehalogenase"
                     /gene="HT2"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="HaloTag®"
     gene            1079..1417
                     /standard_name="optZF_VHH05"
     3'UTR           1439..1761
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1852..1870)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1888..2009
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2035..2067)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2121..2140)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2179..2206)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2185..2201
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2210..2239)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2217..2228
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2254..2275
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2548..3167)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3322..4182)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4183..4287)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4224..4252)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4445..4751)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4840..5826
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cagaaatcgg
      181 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
      241 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
      301 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
      361 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
      421 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
      481 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
      541 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
      601 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
      661 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
      721 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
      781 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
      841 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
      901 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
      961 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1021 gatcgcgcgc tggctgtcga cgctcgagat ttccggcctc gagaggtgtg gtcgacatat
     1081 ggatcaggtg cagctgcagg agtcaggcgg cggactggtg cagcctggag ggtctctacg
     1141 tctgtcctgt gctgcctctg gattcgtctt cgagaattcg gccatggcct ggtaccgtca
     1201 ggctcctgga aaggagcgtg aactgatcgc tgttattggt acgaccttta taaagcttgc
     1261 tgagtccgtg aagggacgat tcaccatctc ccgtgataat gccaagagta cggtctacct
     1321 gcaaatgaat aatctgaagc ctgaggacac cgccgtgtat tactgttcaa agtctggcgc
     1381 ctactgggga cagggaaccc aggtcaccgt ctcctcataa tacgtagatt aagagctctc
     1441 tcaaaatgag gaatgagcaa tgatttttta aagaaaaatt ttacgagttg gattttattt
     1501 cactggtgaa cggagtgcgc ggctacattg aggcttcgag atggtggtca tgcgccacag
     1561 acaaatgtag tttcagtcac tgtttggcta acttattcca cttgggctgt ctgtttagcg
     1621 gcttgccttg tttattacat tattttgcta ccaaaggttt tattgtgaat tgccaatgtt
     1681 ttcatgaatc aagtttgcgt catctttaaa tagttgttta gacattttaa atgttcattg
     1741 ttaataaaaa taactaaaca actagaatac atacttctaa tcattccata gctaatacat
     1801 acttctaatc attccatagc taatacatac ttctaatcat tccatactag aactatagtg
     1861 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1921 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1981 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2041 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg
     2101 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2161 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2221 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2281 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3001 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     4321 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     4381 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     4441 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4501 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4561 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4621 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4681 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4741 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     4801 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc caattcaata
     4861 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata
     4921 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata
     4981 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga
     5041 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc
     5101 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat
     5161 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta
     5221 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc
     5281 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca
     5341 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg
     5401 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct
     5461 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca
     5521 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac
     5581 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaaggac
     5641 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc
     5701 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca
     5761 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta
     5821 gggaac
//
