LOCUS       pCS2+_Halo-Nb127d01_boomUTR        5838 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  30.
ACCESSION   urn.local...ql-klr9qcb
VERSION     urn.local...ql-klr9qcb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    <170..>1063
                     /note="HaloTag used for protein expression and
                     purification"
                     /product="haloalkane dehalogenase"
                     /gene="HT2"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="HaloTag®"
     misc_feature    1063^1064
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TAA"
     gene            1079..1429
                     /standard_name="optZF_Nb127d01"
     3'UTR           1451..1773
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1864..1882)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1900..2021
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2047..2079)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2133..2152)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2191..2218)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2197..2213
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2222..2251)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2229..2240
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2266..2287
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2560..3179)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3334..4194)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4195..4299)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4236..4264)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4457..4763)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4852..5838
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg cagaaatcgg
      181 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
      241 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
      301 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
      361 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
      421 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
      481 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
      541 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
      601 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
      661 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
      721 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
      781 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
      841 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
      901 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
      961 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1021 gatcgcgcgc tggctgtcga cgctcgagat ttccggcctc gagaggtgtg gtcgacatat
     1081 ggatgaggtc caacttgtgg aatctggcgg aggactggtt caagccggtg agtccttgcg
     1141 tttgagctgc gctgcttctg ggagcacctt tgatttcaag gtgatgggct ggtatcgtca
     1201 gccgcctgga aagcagcgtg agggtgtagc tgccatccgc ctgtctggaa atatgcacta
     1261 cgctgagtct gtcaaagggc gctttgctat ttccaaagct aacgctaaga atacagtcta
     1321 ccttcaaatg aattcattgc gtcctgagga taccgctgtt tattactgca aagtcaacat
     1381 ccgtgggcag gattactggg gtcaaggaac gcaggtcacc gtctcttctt aatacgtaga
     1441 ttaagagctc tctcaaaatg aggaatgagc aatgattttt taaagaaaaa ttttacgagt
     1501 tggattttat ttcactggtg aacggagtgc gcggctacat tgaggcttcg agatggtggt
     1561 catgcgccac agacaaatgt agtttcagtc actgtttggc taacttattc cacttgggct
     1621 gtctgtttag cggcttgcct tgtttattac attattttgc taccaaaggt tttattgtga
     1681 attgccaatg ttttcatgaa tcaagtttgc gtcatcttta aatagttgtt tagacatttt
     1741 aaatgttcat tgttaataaa aataactaaa caactagaat acatacttct aatcattcca
     1801 tagctaatac atacttctaa tcattccata gctaatacat acttctaatc attccatact
     1861 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     1921 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1981 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2041 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc
     2101 caatgcattg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     2161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4321 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4381 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4441 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4501 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4561 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4621 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4681 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4741 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     4801 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gtcgaccata
     4861 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
     4921 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
     4981 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ttggcactgt
     5041 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
     5101 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
     5161 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
     5221 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
     5281 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
     5341 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
     5401 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
     5461 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
     5521 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
     5581 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
     5641 tattggaagg acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
     5701 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
     5761 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
     5821 aatgctcgtt tagggaac
//
