LOCUS       pCS2+_Halo-FbHA_boomUTR        6235 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  5.
ACCESSION   urn.local...7j-klr5pz7
VERSION     urn.local...7j-klr5pz7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    <171..>1064
                     /note="HaloTag used for protein expression and
                     purification"
                     /product="haloalkane dehalogenase"
                     /gene="HT2"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="HaloTag®"
     CDS             1065..1841
                     /standard_name="HA Frankenbody (15F11)"
     misc_feature    1064^1065
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TAACAGATCGCCGCCACC"
     misc_feature    1838^1839
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGAGGTTCTGGAGGAGGAGGTTCGGGAGGTGGTGGA
                     TCAGGAGGCGGCGGAAGCGGTGGAGGAGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1848..2170
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2261..2279)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2297..2418
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2444..2476)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2530..2549)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2588..2615)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2594..2610
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2619..2648)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2626..2637
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2663..2684
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2957..3576)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3731..4591)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4592..4696)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4633..4661)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4854..5160)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5249..6235
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gcagaaatcg
      181 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
      241 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
      301 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
      361 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
      421 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
      481 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
      541 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
      601 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
      661 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
      721 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
      781 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtagcgctg gtcgaagaat
      841 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
      901 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
      961 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     1021 agatcgcgcg ctggctgtcg acgctcgaga tttccggcct cgagatggcc gaggtgaagc
     1081 tggtggagtc tggaggaggc ctggtgaagc ctggaggatc cctgaagctc tcctgtgctg
     1141 cctcgggatt cactttctcc tcatacggca tgtcttgggt tagacagact ccggagaagc
     1201 gtcttgagtg ggtcgccact attagtcgag gtggtagtta cacctactat ccagacagtg
     1261 tgaaggggcg attcaccatc tcccgtgaca atgccaagaa caccctgtat ctgcagatga
     1321 gctctctgcg ttctgaggat acggccatct attactgtgc acgacgtgaa acatatgatg
     1381 aaaagggctt tgcctactgg ggccaaggca ccactctcac agtctcctca ggtggaggcg
     1441 gatcaggcgg aggtggatct ggcggtggag gatcggatat cgtgctgacc caatccccag
     1501 cttctttgac tgtgtctctg gggcagaggg ccaccatctc ctgcaaatcc agccaaagtc
     1561 tgctgaatag tggaaatcag aagaactacc tcacctggta ccaacagaaa ccaggacagc
     1621 ctccaaagct cctcatctac tgggcatcca ctcgtgaatc cgggattcca gctaggttca
     1681 gtggcagtgg gtctgggaca gacttcaccc tcaacatcca tcctgtggag gaggaagatg
     1741 ctgctaccta ttactgtcag aacgataatt cccatccgct tacgttcggt gctgggacca
     1801 agctggaaat aaagagagcg gcagccaagg gcgaattcta agagctctct caaaatgagg
     1861 aatgagcaat gattttttaa agaaaaattt tacgagttgg attttatttc actggtgaac
     1921 ggagtgcgcg gctacattga ggcttcgaga tggtggtcat gcgccacaga caaatgtagt
     1981 ttcagtcact gtttggctaa cttattccac ttgggctgtc tgtttagcgg cttgccttgt
     2041 ttattacatt attttgctac caaaggtttt attgtgaatt gccaatgttt tcatgaatca
     2101 agtttgcgtc atctttaaat agttgtttag acattttaaa tgttcattgt taataaaaat
     2161 aactaaacaa ctagaataca tacttctaat cattccatag ctaatacata cttctaatca
     2221 ttccatagct aatacatact tctaatcatt ccatactaga actatagtga gtcgtattac
     2281 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2341 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2401 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2461 gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca
     2521 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     4741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     5041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     5101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     5161 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5221 ggtgcgggcc tcttcgctat tacgccagtc gaccatagcc aattcaatat ggcgtatatg
     5281 gactcatgcc aattcaatat ggtggatctg gacctgtgcc aattcaatat ggcgtatatg
     5341 gactcgtgcc aattcaatat ggtggatctg gaccccagcc aattcaatat ggcggacttg
     5401 gcaccatgcc aattcaatat ggcggacttg gcactgtgcc aactggggag gggtctactt
     5461 ggcacggtgc caagtttgag gaggggtctt ggccctgtgc caagtccgcc atattgaatt
     5521 ggcatggtgc caataatggc ggccatattg gctatatgcc aggatcaata tataggcaat
     5581 atccaatatg gccctatgcc aatatggcta ttggccaggt tcaatactat gtattggccc
     5641 tatgccatat agtattccat atatgggttt tcctattgac gtagatagcc cctcccaatg
     5701 ggcggtccca tataccatat atggggcttc ctaataccgc ccatagccac tcccccattg
     5761 acgtcaatgg tctctatata tggtctttcc tattgacgtc atatgggcgg tcctattgac
     5821 gtatatggcg cctcccccat tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc
     5881 cattgacgtc aataggacca cccaccattg acgtcaatgg gatggctcat tgcccattca
     5941 tatccgttct cacgccccct attgacgtca atgacggtaa atggcccact tggcagtaca
     6001 tcaatatcta ttaatagtaa cttggcaagt acattactat tggaaggacg ccagggtaca
     6061 ttggcagtac tcccattgac gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca
     6121 tccccattga cgtcaatggg gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat
     6181 gggcggtagg cgtgcctaat gggaggtcta tataagcaat gctcgtttag ggaac
//
