LOCUS       pCS2+_Halo-FbFLAG_boomUTR        6226 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  11.
ACCESSION   urn.local...fq-klr7f1f
VERSION     urn.local...fq-klr7f1f
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    <171..>1064
                     /note="HaloTag used for protein expression and
                     purification"
                     /product="haloalkane dehalogenase"
                     /gene="HT2"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="HaloTag®"
     CDS             1065..1829
                     /standard_name="FbFLAG"
     misc_feature    1064^1065
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TAACAGATCGCCGCCACC"
     misc_feature    1829^1830
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGCGGTTCTGGGGGTGGAGGTAGTGGAGGTGGAGGT
                     TCAGGAGGCGGAGGAAGCGGTGGAGGTGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1839..2161
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2252..2270)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2288..2409
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2435..2467)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2521..2540)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2579..2606)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2585..2601
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2610..2639)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2617..2628
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2654..2675
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2948..3567)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3722..4582)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4583..4687)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4624..4652)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4845..5151)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5240..6226
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gcagaaatcg
      181 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
      241 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
      301 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
      361 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
      421 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
      481 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
      541 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
      601 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
      661 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
      721 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
      781 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
      841 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
      901 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
      961 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     1021 agatcgcgcg ctggctgtcg acgctcgaga tttccggcct cgagatggcc gaggtgaagc
     1081 tggtggaatc tggaggagga ttagtgaagc ctggaggttc cctgaagctc tcatgcgcag
     1141 cctctggatt caccttcagt agctttggaa tgcattgggt tagacagact ccggagaagc
     1201 gtcttgagtg ggtagcatac attagtagtg gcagtagtac catctactat gcagacaccg
     1261 tgaagggccg attcaccatc tcccgtgaca atgccaagaa cacgctgtac ctgcaaatga
     1321 gcagtctgag gtctgaggac acggctattt attactgtgc tagatccctt gctaccgccg
     1381 cttttgctta ctggggacaa ggcaccactc tcaccgtttc ctcaggtgga ggcggttcag
     1441 gaggtggagg ctctggcggt ggcggatcgg acattgtgct gacccaatct ccagcatctc
     1501 tgactgtgtc tctagggcag agggccacca tctcctgccg ttctagtcag agtattgtct
     1561 atagtaatgg taacacctac cttgaatggt accaacagaa gccaggacag ccacccaaac
     1621 tcctgatcta taaggtttct aaccgatttt ccggaatccc agccaggttc agtggcagtg
     1681 ggtctgggac agacttcacc ctgaacattc atcctgtgga ggaggaggat gctgctacct
     1741 attactgctt tcagggatca catgttccgt acacattcgg tgctggcacc aagctggaaa
     1801 taaaacgggc tgccgccaag ggcgagttct aagagctctc tcaaaatgag gaatgagcaa
     1861 tgatttttta aagaaaaatt ttacgagttg gattttattt cactggtgaa cggagtgcgc
     1921 ggctacattg aggcttcgag atggtggtca tgcgccacag acaaatgtag tttcagtcac
     1981 tgtttggcta acttattcca cttgggctgt ctgtttagcg gcttgccttg tttattacat
     2041 tattttgcta ccaaaggttt tattgtgaat tgccaatgtt ttcatgaatc aagtttgcgt
     2101 catctttaaa tagttgttta gacattttaa atgttcattg ttaataaaaa taactaaaca
     2161 actagaatac atacttctaa tcattccata gctaatacat acttctaatc attccatagc
     2221 taatacatac ttctaatcat tccatactag aactatagtg agtcgtatta cgtagatcca
     2281 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2341 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2401 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2461 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     2521 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4681 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4741 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4801 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4861 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4921 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4981 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5041 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5101 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5161 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5221 ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc
     5281 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     5341 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     5401 caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg
     5461 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     5521 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     5581 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     5641 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     5701 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     5761 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     5821 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     5881 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     5941 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     6001 attaatagta acttggcaag tacattacta ttggaaggac gccagggtac attggcagta
     6061 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     6121 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     6181 gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
