LOCUS       pCS2+_HAgrad            5986 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  6.
ACCESSION   urn.local...gt-klr7pna
VERSION     urn.local...gt-klr7pna
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     gene            167..817
                     /standard_name="zebrafish fbxw11b"
     misc_feature    817^818
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATGGAGACGGAGATGGAGGACAAAACCTTAGAGCAGATGAAC
                     ACCTCTGTCATGGACCCGCAGACTGCAGACCGCTCTCCAAAGATCACATTGATTAAGT
                     CTACCTTTATCTGTCCTCAGGTGAGTAATGGACCCTTGACGGGCTCTAGGAAGCGGCC
                     GTCAGAGGGAAATTACGAGAAGGAAAAGGACGTGTGCATCCAGCTCTTTGATCAATGG
                     TCTGAGGCCGACCAGGTGGAGTTTGTGGAACACCTGATCTCACGCATGTGCCATTACC
                     AACACGGCCACATAAACTCCTACCTCAAACCCATGCTCCAAAGGGATTTCATTACGGC
                     ACTGCCAGCTCAGGGTCTGGATCATATAGCGGAGAATATCTTGTCGTTCCTGGATGCT
                     CGCTCGTTGTGTTCGGCTGAGCTGGTGTGTAAGGAATGGCAGCGTGTCATATCTGAAG
                     GCATGTTGTGGAAGAAGCTGATTGAGAGGATGGTTCGGACAGACCCGCTGTGGAAAGG
                     CCTATCAGAGAGACACCAGTGGGAAAAATACCTGTTCAAGAACCGCACAACAGAAGTT
                     CCTCCAAACTCGTACTACCGCTCTCTTTATCCAAAAATCATTCAGGACATAGAGACCA
                     TTGAAGCAAACTGGAGGTGTGGTCGACATATGGATCAATATGGATCAAGGAGGCGGAG
                     GTTCTGGGGGAGGAGGTAGTGGCGGTGGTGGTTCAGGAGGCGGCGGAAGCGGTGGAGG
                     TGGAAGCGGTGGAGGCGGAGGTTCTGGGGGAGGAGGTAGTGGCGGTGGTGGTTCAGGA
                     GGCGGCGGAAGCGGTGGAGGTGGAAGCGGT"
     misc_feature    <818..>1588
                     /note="Geneious type: Homology Arm"
                     /standard_name="anti-HA frankenbody"
     3'UTR           1598..1921
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2012..2030)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2048..2169
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2195..2227)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2281..2300)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2339..2366)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2345..2361
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2370..2399)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2377..2388
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2414..2435
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2708..3327)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3482..4342)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4343..4447)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4384..4412)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4605..4911)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5000..5986
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat
      181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg
      241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc
      301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg
      361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat
      421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca
      481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt
      541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg
      601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc
      661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac
      721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat
      781 agagaccatt gaagcaaact ggaggtgtgg tcgacatgcc gaggtgaagc tggtggagtc
      841 tgggggaggc ttagtgaagc ctggagggtc cctgaaactc tcctgtgcag cctctggatt
      901 cactttcagt agttatggca tgtcttgggt tcgccagact ccggagaaga ggctggagtg
      961 ggtcgcaacc attagtagag gtggtagtta tacctactat ccagacagtg tgaaggggcg
     1021 attcaccatc tccagagaca atgccaagaa caccctgtac ctgcaaatga gcagtctgag
     1081 gtctgaggac acggccatat attactgtgc aagacgggaa acatacgatg aaaagggctt
     1141 tgcctactgg ggccaaggca ccactctcac agtctcctca ggtggaggcg gttcaggcgg
     1201 aggtggctct ggcggtggcg gatcggacat tgtgctgacc caatctccag cttctttgac
     1261 tgtgtctcta gggcagaggg ccaccatctc ctgcaagtcc agccaaagtc ttctgaatag
     1321 tggtaatcag aagaactacc tcacctggta ccaacagaaa ccaggacagc cacccaaact
     1381 cctcatctat tgggcatcca ctagggaatc tgggatccca gccaggttta gtggcagtgg
     1441 gtctgggaca gacttcaccc tcaacatcca tcctgtggag gaggaggatg ctgcaaccta
     1501 ttactgtcag aacgataatt cccatccgct cacgttcggt gctgggacca agctggaaat
     1561 aaaacgggcg gccgccaagg gcgaattcta agagctctct caaaatgagg aatgagcaat
     1621 gattttttta aagaaaaatt ttacgagttg gattttattt cactggtgaa cggagtgcgc
     1681 ggctacattg aggcttcgag atggtggtca tgcgccacag acaaatgtag tttcagtcac
     1741 tgtttggcta acttattcca cttgggctgt ctgtttagcg gcttgccttg tttattacat
     1801 tattttgcta ccaaaggttt tattgtgaat tgccaatgtt ttcatgaatc aagtatgcgt
     1861 catctttaaa tagttgttta gacattttaa atgttcattg ttaataaaaa taactaaaca
     1921 actagaatac atacttctaa tcattccata gctaatacat acttctaatc attccatagc
     1981 taatacatac ttctaatcat tccatactag aactatagtg agtcgtatta cgtagatcca
     2041 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2101 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2161 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2221 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     2281 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2341 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2401 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2461 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3121 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4381 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4441 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4501 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4561 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4621 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4681 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4741 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4801 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4861 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4921 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4981 ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc
     5041 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     5101 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     5161 caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg
     5221 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     5281 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     5341 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     5401 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     5461 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     5521 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     5581 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     5641 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     5701 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     5761 attaatagta acttggcaag tacattacta ttggaaggac gccagggtac attggcagta
     5821 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     5881 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     5941 gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
