LOCUS       pCS2+_FLAGrad           5977 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  12.
ACCESSION   urn.local...gl-klr7p2s
VERSION     urn.local...gl-klr7p2s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     gene            167..815
                     /standard_name="zebrafish fbxw11b"
     CDS             816..1580
                     /standard_name="FbFLAG"
     misc_feature    1580^1581
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGCGGTTCTGGGGGTGGAGGTAGTGGAGGTGGAGGT
                     TCAGGAGGCGGAGGAAGCGGTGGAGGTGGAAGC"
     3'UTR           1590..1912
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2003..2021)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2039..2160
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2186..2218)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2272..2291)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2330..2357)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2336..2352
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2361..2390)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2368..2379
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2405..2426
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2699..3318)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3473..4333)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4334..4438)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4375..4403)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4596..4902)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4991..5977
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat
      181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg
      241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc
      301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg
      361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat
      421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca
      481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt
      541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg
      601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc
      661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac
      721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat
      781 agagaccatt gaagcaaact ggaggtgtgg tcgacatggc cgaggtgaag ctggtggaat
      841 ctggaggagg attagtgaag cctggaggtt ccctgaagct ctcatgcgca gcctctggat
      901 tcaccttcag tagctttgga atgcattggg ttagacagac tccggagaag cgtcttgagt
      961 gggtagcata cattagtagt ggcagtagta ccatctacta tgcagacacc gtgaagggcc
     1021 gattcaccat ctcccgtgac aatgccaaga acacgctgta cctgcaaatg agcagtctga
     1081 ggtctgagga cacggctatt tattactgtg ctagatccct tgctaccgcc gcttttgctt
     1141 actggggaca aggcaccact ctcaccgttt cctcaggtgg aggcggttca ggaggtggag
     1201 gctctggcgg tggcggatcg gacattgtgc tgacccaatc tccagcatct ctgactgtgt
     1261 ctctagggca gagggccacc atctcctgcc gttctagtca gagtattgtc tatagtaatg
     1321 gtaacaccta ccttgaatgg taccaacaga agccaggaca gccacccaaa ctcctgatct
     1381 ataaggtttc taaccgattt tccggaatcc cagccaggtt cagtggcagt gggtctggga
     1441 cagacttcac cctgaacatt catcctgtgg aggaggagga tgctgctacc tattactgct
     1501 ttcagggatc acatgttccg tacacattcg gtgctggcac caagctggaa ataaaacggg
     1561 ctgccgccaa gggcgagttc taagagctct ctcaaaatga ggaatgagca atgatttttt
     1621 aaagaaaaat tttacgagtt ggattttatt tcactggtga acggagtgcg cggctacatt
     1681 gaggcttcga gatggtggtc atgcgccaca gacaaatgta gtttcagtca ctgtttggct
     1741 aacttattcc acttgggctg tctgtttagc ggcttgcctt gtttattaca ttattttgct
     1801 accaaaggtt ttattgtgaa ttgccaatgt tttcatgaat caagtttgcg tcatctttaa
     1861 atagttgttt agacatttta aatgttcatt gttaataaaa ataactaaac aactagaata
     1921 catacttcta atcattccat agctaataca tacttctaat cattccatag ctaatacata
     1981 cttctaatca ttccatacta gaactatagt gagtcgtatt acgtagatcc agacatgata
     2041 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2101 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2161 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2221 taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt tccctttagt
     2281 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3121 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4441 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     4501 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4561 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4621 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4681 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4741 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4801 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4861 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4921 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4981 attacgccag tcgaccatag ccaattcaat atggcgtata tggactcatg ccaattcaat
     5041 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
     5101 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
     5161 atggcggact tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
     5221 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
     5281 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
     5341 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
     5401 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
     5461 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
     5521 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
     5581 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
     5641 cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc
     5701 ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt
     5761 aacttggcaa gtacattact attggaagga cgccagggta cattggcagt actcccattg
     5821 acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg
     5881 gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta
     5941 atgggaggtc tatataagca atgctcgttt agggaac
//
