LOCUS       pCS2+_EGFP-NbVHH05_boomUTR        5646 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  20.
ACCESSION   urn.local...4d-klr57vw
VERSION     urn.local...4d-klr57vw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..883
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     gene            899..1237
                     /standard_name="optZF_VHH05"
     3'UTR           1259..1581
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1672..1690)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1708..1829
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1855..1887)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1941..1960)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1999..2026)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2005..2021
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2030..2059)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2037..2048
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2074..2095
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2368..2987)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3142..4002)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4003..4107)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4044..4072)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4265..4571)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4660..5646
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggtgtg gtcgacatat
      901 ggatcaggtg cagctgcagg agtcaggcgg cggactggtg cagcctggag ggtctctacg
      961 tctgtcctgt gctgcctctg gattcgtctt cgagaattcg gccatggcct ggtaccgtca
     1021 ggctcctgga aaggagcgtg aactgatcgc tgttattggt acgaccttta taaagcttgc
     1081 tgagtccgtg aagggacgat tcaccatctc ccgtgataat gccaagagta cggtctacct
     1141 gcaaatgaat aatctgaagc ctgaggacac cgccgtgtat tactgttcaa agtctggcgc
     1201 ctactgggga cagggaaccc aggtcaccgt ctcctcataa tacgtagatt aagagctctc
     1261 tcaaaatgag gaatgagcaa tgatttttta aagaaaaatt ttacgagttg gattttattt
     1321 cactggtgaa cggagtgcgc ggctacattg aggcttcgag atggtggtca tgcgccacag
     1381 acaaatgtag tttcagtcac tgtttggcta acttattcca cttgggctgt ctgtttagcg
     1441 gcttgccttg tttattacat tattttgcta ccaaaggttt tattgtgaat tgccaatgtt
     1501 ttcatgaatc aagtttgcgt catctttaaa tagttgttta gacattttaa atgttcattg
     1561 ttaataaaaa taactaaaca actagaatac atacttctaa tcattccata gctaatacat
     1621 acttctaatc attccatagc taatacatac ttctaatcat tccatactag aactatagtg
     1681 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1741 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1801 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1861 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg
     1921 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     1981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2041 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2101 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2821 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2941 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3061 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3121 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3181 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3241 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3301 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3361 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3421 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3481 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3541 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3601 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3661 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3721 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3781 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3841 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3901 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3961 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4021 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4081 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     4141 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     4201 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     4261 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4321 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4381 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4441 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4501 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4561 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     4621 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc caattcaata
     4681 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata
     4741 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata
     4801 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga
     4861 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc
     4921 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat
     4981 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta
     5041 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc
     5101 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca
     5161 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg
     5221 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct
     5281 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca
     5341 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac
     5401 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaaggac
     5461 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc
     5521 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca
     5581 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta
     5641 gggaac
//
