LOCUS       pCS2+_EGFP-NbMoon_boomUTR        5655 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  34.
ACCESSION   urn.local...rj-klr9txw
VERSION     urn.local...rj-klr9txw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..883
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     CDS             884..1258
                     /standard_name="NbMoon (gp41 nanobody)"
     3'UTR           1268..1590
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1681..1699)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1717..1838
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1864..1896)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1950..1969)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2008..2035)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2014..2030
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2039..2068)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2046..2057
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2083..2104
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2377..2996)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3151..4011)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4012..4116)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     misc_feature    complement(4274..4580)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4669..5655
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagatggagg tgcagctggt
      901 ggaatctggg ggcggactgg tgcagcccgg gggatctctg cggctgtcct gcgccgcctc
      961 tggctccatc tctagcgtgg atgtgatgtc ctggtacagg caggcccctg gcaagcagag
     1021 ggaactggtg gccttcatta ctgatagggg aagaaccaat tacaaagtga gcgtgaaagg
     1081 ccgcttcact atcagccggg ataattctaa gaatatggtg tatctgcaga tgaacagcct
     1141 gaagccagaa gacactgccg actatctgtg cagggctgag tcccggactt cctggtccag
     1201 cccatctcct ctggatgtgt gggggcgcgg cactcaggtg actgtgtcct cctggtgata
     1261 agagctctct caaaatgagg aatgagcaat gattttttaa agaaaaattt tacgagttgg
     1321 attttatttc actggtgaac ggagtgcgcg gctacattga ggcttcgaga tggtggtcat
     1381 gcgccacaga caaatgtagt ttcagtcact gtttggctaa cttattccac ttgggctgtc
     1441 tgtttagcgg cttgccttgt ttattacatt attttgctac caaaggtttt attgtgaatt
     1501 gccaatgttt tcatgaatca agtttgcgtc atctttaaat agttgtttag acattttaaa
     1561 tgttcattgt taataaaaat aactaaacaa ctagaataca tacttctaat cattccatag
     1621 ctaatacata cttctaatca ttccatagct aatacatact tctaatcatt ccatactaga
     1681 actatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
     1741 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1801 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1861 ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcggcgccaa
     1921 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     1981 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2041 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2101 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2161 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2821 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4141 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4201 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4261 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4321 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4381 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4441 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4501 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4561 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     4621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gaccatagcc
     4681 aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg gacctgtgcc
     4741 aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg gaccccagcc
     4801 aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacttg gcactgtgcc
     4861 aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt ggccctgtgc
     4921 caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg gctatatgcc
     4981 aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta ttggccaggt
     5041 tcaatactat gtattggccc tatgccatat agtattccat atatgggttt tcctattgac
     5101 gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc ctaataccgc
     5161 ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc tattgacgtc
     5221 atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat tacggtaaat
     5281 ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg acgtcaatgg
     5341 gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca atgacggtaa
     5401 atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt acattactat
     5461 tggaaggacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg gtaaatggcc
     5521 cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat gacgcaaatg
     5581 ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta tataagcaat
     5641 gctcgtttag ggaac
//
