LOCUS       pCS2+_EGFP-NbALFA_boomUTR        5679 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  13.
ACCESSION   urn.local...36-klr53vb
VERSION     urn.local...36-klr53vb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..883
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     gene            899..1270
                     /standard_name="optZF_NbALFA"
     3'UTR           1292..1614
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1705..1723)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1741..1862
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1888..1920)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1974..1993)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2032..2059)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2038..2054
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2063..2092)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2070..2081
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2107..2128
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(2416..3004)
     CDS             complement(3175..4035)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(4036..4140)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4077..4105)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     rep_origin      complement(4166..4621)
     regulatory      4693..5679
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggtgtg gtcgacatat
      901 ggatgaagtc cagctgcagg agtccggtgg tggcctggtt caaccaggtg gctccctacg
      961 actgtcctgt accgcatccg gagttacgat tagcgctctg aatgctatgg caatgggatg
     1021 gtatcgtcag gcaccgggag agcgtcgtgt catggtagct gctgtctctg aacgtggaaa
     1081 tgctatgtat cgtgaatcag ttcagggccg ttttaccgtt acccgcgatt tcaccaacaa
     1141 aatggtcagt ctgcagatgg acaacctgaa accggaggat acagcggtgt actactgcca
     1201 tgtcctggaa gatcgcgttg atagcttcca cgattattgg ggacagggga cccaggtcac
     1261 tgtgtcctca taatacgtag attaagagct ctctcaaaat gaggaatgag caatgatttt
     1321 ttaaagaaaa attttacgag ttggatttta tttcactggt gaacggagtg cgcggctaca
     1381 ttgaggcttc gagatggtgg tcatgcgcca cagacaaatg tagtttcagt cactgtttgg
     1441 ctaacttatt ccacttgggc tgtctgttta gcggcttgcc ttgtttatta cattattttg
     1501 ctaccaaagg ttttattgtg aattgccaat gttttcatga atcaagtttg cgtcatcttt
     1561 aaatagttgt ttagacattt taaatgttca ttgttaataa aaataactaa acaactagaa
     1621 tacatacttc taatcattcc atagctaata catacttcta atcattccat agctaataca
     1681 tacttctaat cattccatac tagaactata gtgagtcgta ttacgtagat ccagacatga
     1741 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1801 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1861 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     1921 tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta
     1981 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2821 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     2881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4141 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     4201 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4261 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4321 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4381 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4441 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     4501 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4561 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4621 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4681 ctattacgcc agtcgaccat agccaattca atatggcgta tatggactca tgccaattca
     4741 atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca
     4801 atatggtgga tctggacccc agccaattca atatggcgga cttggcacca tgccaattca
     4861 atatggcgga cttggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt
     4921 tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa
     4981 tggcggccat attggctata tgccaggatc aatatatagg caatatccaa tatggcccta
     5041 tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt
     5101 ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc
     5161 atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta
     5221 tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc
     5281 ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg
     5341 accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc
     5401 ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata
     5461 gtaacttggc aagtacatta ctattggaag gacgccaggg tacattggca gtactcccat
     5521 tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa
     5581 tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc
     5641 taatgggagg tctatataag caatgctcgt ttagggaac
//
