LOCUS       pCS2+_EGFP-Nb127d01_boomUTR        5658 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  26.
ACCESSION   urn.local...35-klr53vb
VERSION     urn.local...35-klr53vb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..883
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     gene            899..1249
                     /standard_name="optZF_Nb127d01"
     3'UTR           1271..1593
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1684..1702)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1720..1841
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1867..1899)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1953..1972)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2011..2038)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2017..2033
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2042..2071)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2049..2060
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2086..2107
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2380..2999)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3154..4014)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4015..4119)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4056..4084)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4277..4583)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4672..5658
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggtgtg gtcgacatat
      901 ggatgaggtc caacttgtgg aatctggcgg aggactggtt caagccggtg agtccttgcg
      961 tttgagctgc gctgcttctg ggagcacctt tgatttcaag gtgatgggct ggtatcgtca
     1021 gccgcctgga aagcagcgtg agggtgtagc tgccatccgc ctgtctggaa atatgcacta
     1081 cgctgagtct gtcaaagggc gctttgctat ttccaaagct aacgctaaga atacagtcta
     1141 ccttcaaatg aattcattgc gtcctgagga taccgctgtt tattactgca aagtcaacat
     1201 ccgtgggcag gattactggg gtcaaggaac gcaggtcacc gtctcttctt aatacgtaga
     1261 ttaagagctc tctcaaaatg aggaatgagc aatgattttt taaagaaaaa ttttacgagt
     1321 tggattttat ttcactggtg aacggagtgc gcggctacat tgaggcttcg agatggtggt
     1381 catgcgccac agacaaatgt agtttcagtc actgtttggc taacttattc cacttgggct
     1441 gtctgtttag cggcttgcct tgtttattac attattttgc taccaaaggt tttattgtga
     1501 attgccaatg ttttcatgaa tcaagtttgc gtcatcttta aatagttgtt tagacatttt
     1561 aaatgttcat tgttaataaa aataactaaa caactagaat acatacttct aatcattcca
     1621 tagctaatac atacttctaa tcattccata gctaatacat acttctaatc attccatact
     1681 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     1741 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1801 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1861 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc
     1921 caatgcattg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     1981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2821 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4141 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4201 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4261 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4321 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4381 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4441 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4501 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4561 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     4621 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gtcgaccata
     4681 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
     4741 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
     4801 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ttggcactgt
     4861 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
     4921 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
     4981 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
     5041 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
     5101 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
     5161 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
     5221 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
     5281 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
     5341 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
     5401 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
     5461 tattggaagg acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
     5521 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
     5581 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
     5641 aatgctcgtt tagggaac
//
