LOCUS       pCS2+_EGFP-FbSun_boomUTR        6024 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  32.
ACCESSION   urn.local...rk-klr9txw
VERSION     urn.local...rk-klr9txw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             167..883
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     CDS             884..1627
                     /standard_name="FbSun (GCN4 scFv)"
     3'UTR           1637..1959
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2050..2068)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2086..2207
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2233..2265)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2319..2338)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2377..2404)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2383..2399
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2408..2437)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2415..2426
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2452..2473
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2746..3365)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3520..4380)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4381..4485)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4422..4450)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4643..4949)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5038..6024
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagatgggcc ccgacatcgt
      901 gatgacccag agccccagca gcctgagcgc cagcgtgggc gaccgcgtga ccatcacctg
      961 ccgcagcagc accggcgccg tgaccaccag caactacgcc agctgggtgc aggagaagcc
     1021 cggcaagctg ttcaagggcc tgatcggcgg caccaacaac cgcgcccccg gcgtgcccag
     1081 ccgcttcagc ggcagcctga tcggcgacaa ggccaccctg accatcagca gcctgcagcc
     1141 cgaggacttc gccacctact tctgcgccct gtggtacagc aaccactggg tgttcggcca
     1201 gggcaccaag gtggagctga agcgcggcgg cggcggcagc ggcggcggcg gcagcggcgg
     1261 cggcggcagc agcggcggcg gcagcgaggt gaagctgctg gagagcggcg gcggcctggt
     1321 gcagcccggc ggcagcctga agctgagctg cgccgtgagc ggcttcagcc tgaccgacta
     1381 cggcgtgaac tgggtgcgcc aggcccccgg ccgcggcctg gagtggatcg gcgtgatctg
     1441 gggcgacggc atcaccgact acaacagcgc cctgaaggac cgcttcatca tcagcaagga
     1501 caacggcaag aacaccgtgt acctgcagat gagcaaggtg cgcagcgacg acaccgccct
     1561 gtactactgc gtgaccggcc tgttcgacta ctggggccag ggcaccctgg tgaccgtgag
     1621 cagctaataa gagctctctc aaaatgagga atgagcaatg attttttaaa gaaaaatttt
     1681 acgagttgga ttttatttca ctggtgaacg gagtgcgcgg ctacattgag gcttcgagat
     1741 ggtggtcatg cgccacagac aaatgtagtt tcagtcactg tttggctaac ttattccact
     1801 tgggctgtct gtttagcggc ttgccttgtt tattacatta ttttgctacc aaaggtttta
     1861 ttgtgaattg ccaatgtttt catgaatcaa gtttgcgtca tctttaaata gttgtttaga
     1921 cattttaaat gttcattgtt aataaaaata actaaacaac tagaatacat acttctaatc
     1981 attccatagc taatacatac ttctaatcat tccatagcta atacatactt ctaatcattc
     2041 catactagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     2101 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2161 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2221 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg
     2281 cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     2341 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2401 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2461 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2521 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3181 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4501 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4561 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     4621 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4681 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4741 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4801 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4861 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4921 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     4981 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg
     5041 accatagcca attcaatatg gcgtatatgg actcatgcca attcaatatg gtggatctgg
     5101 acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg gtggatctgg
     5161 accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg gcggacttgg
     5221 cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg aggggtcttg
     5281 gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg gccatattgg
     5341 ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca atatggctat
     5401 tggccaggtt caatactatg tattggccct atgccatata gtattccata tatgggtttt
     5461 cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata tggggcttcc
     5521 taataccgcc catagccact cccccattga cgtcaatggt ctctatatat ggtctttcct
     5581 attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt gacgtcaatt
     5641 acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac ccaccattga
     5701 cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta ttgacgtcaa
     5761 tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac ttggcaagta
     5821 cattactatt ggaaggacgc cagggtacat tggcagtact cccattgacg tcaatggcgg
     5881 taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg aggggcaatg
     5941 acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat
     6001 ataagcaatg ctcgtttagg gaac
//
