LOCUS       pCS2+EGFP-FbHA_boomUTR        6055 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  1.
ACCESSION   urn.local...4v-klr5eon
VERSION     urn.local...4v-klr5eon
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             168..884
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     CDS             885..1661
                     /standard_name="HA Frankenbody (15F11)"
     misc_feature    884^885
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     3'UTR           1668..1990
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2081..2099)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2117..2238
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2264..2296)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2350..2369)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2408..2435)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2414..2430
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2439..2468)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2446..2457
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2483..2504
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2777..3396)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3551..4411)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4412..4516)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4453..4481)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4674..4980)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5069..6055
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gtgagcaagg
      181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatggcc gaggtgaagc
      901 tggtggagtc tggaggaggc ctggtgaagc ctggaggatc cctgaagctc tcctgtgctg
      961 cctcgggatt cactttctcc tcatacggca tgtcttgggt tagacagact ccggagaagc
     1021 gtcttgagtg ggtcgccact attagtcgag gtggtagtta cacctactat ccagacagtg
     1081 tgaaggggcg attcaccatc tcccgtgaca atgccaagaa caccctgtat ctgcagatga
     1141 gctctctgcg ttctgaggat acggccatct attactgtgc acgacgtgaa acatatgatg
     1201 aaaagggctt tgcctactgg ggccaaggca ccactctcac agtctcctca ggtggaggcg
     1261 gatcaggcgg aggtggatct ggcggtggag gatcggatat cgtgctgacc caatccccag
     1321 cttctttgac tgtgtctctg gggcagaggg ccaccatctc ctgcaaatcc agccaaagtc
     1381 tgctgaatag tggaaatcag aagaactacc tcacctggta ccaacagaaa ccaggacagc
     1441 ctccaaagct cctcatctac tgggcatcca ctcgtgaatc cgggattcca gctaggttca
     1501 gtggcagtgg gtctgggaca gacttcaccc tcaacatcca tcctgtggag gaggaagatg
     1561 ctgctaccta ttactgtcag aacgataatt cccatccgct tacgttcggt gctgggacca
     1621 agctggaaat aaagagagcg gcagccaagg gcgaattcta agagctctct caaaatgagg
     1681 aatgagcaat gattttttaa agaaaaattt tacgagttgg attttatttc actggtgaac
     1741 ggagtgcgcg gctacattga ggcttcgaga tggtggtcat gcgccacaga caaatgtagt
     1801 ttcagtcact gtttggctaa cttattccac ttgggctgtc tgtttagcgg cttgccttgt
     1861 ttattacatt attttgctac caaaggtttt attgtgaatt gccaatgttt tcatgaatca
     1921 agtttgcgtc atctttaaat agttgtttag acattttaaa tgttcattgt taataaaaat
     1981 aactaaacaa ctagaataca tacttctaat cattccatag ctaatacata cttctaatca
     2041 ttccatagct aatacatact tctaatcatt ccatactaga actatagtga gtcgtattac
     2101 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2161 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2221 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2281 gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca
     2341 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2401 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2461 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2521 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2581 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     4561 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4621 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4681 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4741 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4801 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4861 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4921 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4981 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5041 ggtgcgggcc tcttcgctat tacgccagtc gaccatagcc aattcaatat ggcgtatatg
     5101 gactcatgcc aattcaatat ggtggatctg gacctgtgcc aattcaatat ggcgtatatg
     5161 gactcgtgcc aattcaatat ggtggatctg gaccccagcc aattcaatat ggcggacttg
     5221 gcaccatgcc aattcaatat ggcggacttg gcactgtgcc aactggggag gggtctactt
     5281 ggcacggtgc caagtttgag gaggggtctt ggccctgtgc caagtccgcc atattgaatt
     5341 ggcatggtgc caataatggc ggccatattg gctatatgcc aggatcaata tataggcaat
     5401 atccaatatg gccctatgcc aatatggcta ttggccaggt tcaatactat gtattggccc
     5461 tatgccatat agtattccat atatgggttt tcctattgac gtagatagcc cctcccaatg
     5521 ggcggtccca tataccatat atggggcttc ctaataccgc ccatagccac tcccccattg
     5581 acgtcaatgg tctctatata tggtctttcc tattgacgtc atatgggcgg tcctattgac
     5641 gtatatggcg cctcccccat tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc
     5701 cattgacgtc aataggacca cccaccattg acgtcaatgg gatggctcat tgcccattca
     5761 tatccgttct cacgccccct attgacgtca atgacggtaa atggcccact tggcagtaca
     5821 tcaatatcta ttaatagtaa cttggcaagt acattactat tggaaggacg ccagggtaca
     5881 ttggcagtac tcccattgac gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca
     5941 tccccattga cgtcaatggg gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat
     6001 gggcggtagg cgtgcctaat gggaggtcta tataagcaat gctcgtttag ggaac
//
