LOCUS       pCS2+_EGFP-FbFLAG_boomUTR        6046 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  7.
ACCESSION   urn.local...g3-klr7iv4
VERSION     urn.local...g3-klr7iv4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           110..152
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     CDS             168..884
                     /note="Enhanced green fluorescent reporter protein -
                     Ex:488, Em:507"
                     /product="EGFP"
                     /gene="EGFP"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EGFP"
     CDS             885..1649
                     /standard_name="FbFLAG"
     misc_feature    884^885
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CAGATCGCCGCCACC"
     misc_feature    1649^1650
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GGAGGCGGCGGTTCTGGGGGTGGAGGTAGTGGAGGTGGAGGT
                     TCAGGAGGCGGAGGAAGCGGTGGAGGTGGAAGCGCCTCCCTCCCAGCC"
     3'UTR           1659..1981
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(2072..2090)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      2108..2229
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(2255..2287)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(2341..2360)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(2399..2426)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    2405..2421
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(2430..2459)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    2437..2448
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      2474..2495
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2768..3387)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3542..4402)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(4403..4507)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(4444..4472)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4665..4971)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      5060..6046
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca aggagcttgc ttgttctttt
      121 tgcagaagct cagaataaac gctcaacttt ggcagatcgc cgccaccatg gtgagcaagg
      181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatggcc gaggtgaagc
      901 tggtggaatc tggaggagga ttagtgaagc ctggaggttc cctgaagctc tcatgcgcag
      961 cctctggatt caccttcagt agctttggaa tgcattgggt tagacagact ccggagaagc
     1021 gtcttgagtg ggtagcatac attagtagtg gcagtagtac catctactat gcagacaccg
     1081 tgaagggccg attcaccatc tcccgtgaca atgccaagaa cacgctgtac ctgcaaatga
     1141 gcagtctgag gtctgaggac acggctattt attactgtgc tagatccctt gctaccgccg
     1201 cttttgctta ctggggacaa ggcaccactc tcaccgtttc ctcaggtgga ggcggttcag
     1261 gaggtggagg ctctggcggt ggcggatcgg acattgtgct gacccaatct ccagcatctc
     1321 tgactgtgtc tctagggcag agggccacca tctcctgccg ttctagtcag agtattgtct
     1381 atagtaatgg taacacctac cttgaatggt accaacagaa gccaggacag ccacccaaac
     1441 tcctgatcta taaggtttct aaccgatttt ccggaatccc agccaggttc agtggcagtg
     1501 ggtctgggac agacttcacc ctgaacattc atcctgtgga ggaggaggat gctgctacct
     1561 attactgctt tcagggatca catgttccgt acacattcgg tgctggcacc aagctggaaa
     1621 taaaacgggc tgccgccaag ggcgagttct aagagctctc tcaaaatgag gaatgagcaa
     1681 tgatttttta aagaaaaatt ttacgagttg gattttattt cactggtgaa cggagtgcgc
     1741 ggctacattg aggcttcgag atggtggtca tgcgccacag acaaatgtag tttcagtcac
     1801 tgtttggcta acttattcca cttgggctgt ctgtttagcg gcttgccttg tttattacat
     1861 tattttgcta ccaaaggttt tattgtgaat tgccaatgtt ttcatgaatc aagtttgcgt
     1921 catctttaaa tagttgttta gacattttaa atgttcattg ttaataaaaa taactaaaca
     1981 actagaatac atacttctaa tcattccata gctaatacat acttctaatc attccatagc
     2041 taatacatac ttctaatcat tccatactag aactatagtg agtcgtatta cgtagatcca
     2101 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2161 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2221 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2281 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     2341 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2401 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2461 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2521 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2581 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3841 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4141 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4501 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4561 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4621 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4681 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4741 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4801 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4861 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4921 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4981 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5041 ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc
     5101 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     5161 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     5221 caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg
     5281 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     5341 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     5401 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     5461 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     5521 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     5581 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     5641 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     5701 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     5761 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     5821 attaatagta acttggcaag tacattacta ttggaaggac gccagggtac attggcagta
     5881 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     5941 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     6001 gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
