LOCUS       pCS2+_127d01Grad        5568 bp    DNA     circular UNA 11-MAY-2026
DEFINITION  31.
ACCESSION   urn.local...rl-klr9txw
VERSION     urn.local...rl-klr9txw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      34..51
                     /note="Geneious type: Promoter"
                     /standard_name="Sp6 prom"
     5'UTR           109..151
                     /note="Xenopus globin 5′ UTR sequence"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Xenopus globin 5′ UTR"
     misc_feature    152..166
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            167..817
                     /standard_name="zebrafish fbxw11b"
     gene            818..1168
                     /standard_name="optZF_Nb127d01"
     3'UTR           1181..1503
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="prrg2 3'UTR"
     regulatory      complement(1594..1612)
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     regulatory      1630..1751
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(1777..1809)
                     /note="KT3 epitope tag used for protein detection and
                     purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="KT3"
     regulatory      complement(1863..1882)
                     /note="Geneious type: Promoter"
                     /standard_name="T3 prom"
     misc_feature    complement(1921..1948)
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="lacO reg"
     misc_feature    1927..1943
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(1952..1981)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    1959..1970
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      1996..2017
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     misc_feature    complement(2290..2909)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3064..3924)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     regulatory      complement(3925..4029)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     regulatory      complement(3966..3994)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    complement(4187..4493)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     regulatory      4582..5568
                     /note="Geneious type: promoter"
                     /note="Human cytomegalovirus (CMV) immediate early
                     promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2026.0.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV IE94"
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaga atcccatcga ttcgaattca agagcttgct tgttcttttt
      121 gcagaagctc agaataaacg ctcaactttg gcagatcgcc gccaccatgg agacggagat
      181 ggaggacaaa accttagagc agatgaacac ctctgtcatg gacccgcaga ctgcagaccg
      241 ctctccaaag atcacattga ttaagtctac ctttatctgt cctcaggtga gtaatggacc
      301 cttgacgggc tctaggaagc ggccgtcaga gggaaattac gagaaggaaa aggacgtgtg
      361 catccagctc tttgatcaat ggtctgaggc cgaccaggtg gagtttgtgg aacacctgat
      421 ctcacgcatg tgccattacc aacacggcca cataaactcc tacctcaaac ccatgctcca
      481 aagggatttc attacggcac tgccagctca gggtctggat catatagcgg agaatatctt
      541 gtcgttcctg gatgctcgct cgttgtgttc ggctgagctg gtgtgtaagg aatggcagcg
      601 tgtcatatct gaaggcatgt tgtggaagaa gctgattgag aggatggttc ggacagaccc
      661 gctgtggaaa ggcctatcag agagacacca gtgggaaaaa tacctgttca agaaccgcac
      721 aacagaagtt cctccaaact cgtactaccg ctctctttat ccaaaaatca ttcaggacat
      781 agagaccatt gaagcaaact ggaggtgtgg tcgacatatg gatgaggtcc aacttgtgga
      841 atctggcgga ggactggttc aagccggtga gtccttgcgt ttgagctgcg ctgcttctgg
      901 gagcaccttt gatttcaagg tgatgggctg gtatcgtcag ccgcctggaa agcagcgtga
      961 gggtgtagct gccatccgcc tgtctggaaa tatgcactac gctgagtctg tcaaagggcg
     1021 ctttgctatt tccaaagcta acgctaagaa tacagtctac cttcaaatga attcattgcg
     1081 tcctgaggat accgctgttt attactgcaa agtcaacatc cgtgggcagg attactgggg
     1141 tcaaggaacg caggtcaccg tctcttctta ataagagctc tctcaaaatg aggaatgagc
     1201 aatgattttt taaagaaaaa ttttacgagt tggattttat ttcactggtg aacggagtgc
     1261 gcggctacat tgaggcttcg agatggtggt catgcgccac agacaaatgt agtttcagtc
     1321 actgtttggc taacttattc cacttgggct gtctgtttag cggcttgcct tgtttattac
     1381 attattttgc taccaaaggt tttattgtga attgccaatg ttttcatgaa tcaagtttgc
     1441 gtcatcttta aatagttgtt tagacatttt aaatgttcat tgttaataaa aataactaaa
     1501 caactagaat acatacttct aatcattcca tagctaatac atacttctaa tcattccata
     1561 gctaatacat acttctaatc attccatact agaactatag tgagtcgtat tacgtagatc
     1621 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1681 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1741 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     1801 gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg
     1861 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     1921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4021 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     4081 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4141 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4201 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4261 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4321 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4381 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4441 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4501 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4561 gcctcttcgc tattacgcca gtcgaccata gccaattcaa tatggcgtat atggactcat
     4621 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
     4681 gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat
     4741 gccaattcaa tatggcggac ttggcactgt gccaactggg gaggggtcta cttggcacgg
     4801 tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg
     4861 tgccaataat ggcggccata ttggctatat gccaggatca atatataggc aatatccaat
     4921 atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca
     4981 tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc
     5041 ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa
     5101 tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg
     5161 gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac
     5221 gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt
     5281 tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat
     5341 ctattaatag taacttggca agtacattac tattggaagg acgccagggt acattggcag
     5401 tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat
     5461 tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt
     5521 aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
